Summary of study ST000110

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000102. The data can be accessed directly via it's Project DOI: 10.21228/M88C7T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000110
Study TitleSIRM Analysis of human P493 cells under hypoxia in [U-13C/15N] labeled Glutamine medium (Both positive and ion mode FTMS)
Study Typetracer
Study SummarySIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in [U-13C/15N] labeled Glutamine medium
Institute
University of Kentucky
DepartmentToxicology
Last NameFan
First NameTeresa
Emailtwmfan@gmail.com
Submit Date2014-09-29
Num Groups4
Total Subjects8
Study CommentsFor protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., Bose, S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. L.J., Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., and Dang, C.V. Cell Metabolism 15, 110 (2012).
Raw Data AvailableNo
Analysis Type DetailGC/LC-MS
Release Date2015-02-03
Release Version1
Teresa Fan Teresa Fan
https://dx.doi.org/10.21228/M88C7T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000102
Project DOI:doi: 10.21228/M88C7T
Project Title:SIRM Analysis of human P493
Project Summary:SIRM Analysis of human MYC-inducible P493 cells
Institute:Johns Hopkins University
Department:Pathology
Last Name:Dang
First Name:Chi
Email:cvdang@jhmi.edu

Subject:

Subject ID:SU000129
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:P493
Species Group:Human

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Oxygen Condition Treatment
SA006052hypox-13C/15N-Gln-plate-1hypoxia None
SA006053hypox-13C/15N-Gln-plate-2hypoxia None
SA006054tet-hypox-13C/15N-Gln-plate-1hypoxia Tetracycline
SA006055tet-hypox-13C/15N-Gln-plate-2hypoxia Tetracycline
SA006056normox-13C/15N-Gln-plate-2normoxia None
SA006057normox-13C/15N-Gln-plate-1normoxia None
SA006058tet-normox-13C/15N-Gln-plate-2normoxia Tetracycline
SA006059tet-normox-13C/15N-Gln-plate-1normoxia Tetracycline
Showing results 1 to 8 of 8

Collection:

Collection ID:CO000113
Collection Protocol Comments:Collection and quench of cells in tissue culture
Sample Type:B Cells

Treatment:

Treatment ID:TR000131
Treatment Protocol Comments:hypox-13C/15N-Gln: cell culture under hypoxia for 24hr + 24hr with [U-13C/15N]-Gln; tet-hypox-13C/15N-Gln: cell culture under hypoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; tet-normox-13C/15N-Gln: cell culture under normoxia with 1ug/ml Tetracycline for 24hr + 24hr with [U-13C/15N]-Gln; normox-13C/15N-Gln: cell culture under normoxia for 24hr + 24hr with [U-13C/15N]-Gln

Sample Preparation:

Sampleprep ID:SP000126
Sampleprep Protocol Comments:Protein extraction and quantification. |Preparation of polar samples for GCMS and NMR. | Lipid extraction and preparation for FTMS.

Combined analysis:

Analysis ID AN000183 AN000184 AN000185
Analysis type MS MS MS
Chromatography type GC GC GC
Chromatography system
Column
MS Type ESI ESI EI
MS instrument type Single quadrupole Single quadrupole GC-ITQ
MS instrument name Thermo LTQ-FT Thermo LTQ-FT Thermo ITQ
Ion Mode POSITIVE NEGATIVE POSITIVE
Units Peak area Peak area Peak area

Chromatography:

Chromatography ID:CH000124
Chromatography Type:GC

MS:

MS ID:MS000146
Analysis ID:AN000183
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000147
Analysis ID:AN000184
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000148
Analysis ID:AN000185
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
Ion Mode:POSITIVE
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