Summary of study ST000121

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000108. The data can be accessed directly via it's Project DOI: 10.21228/M8GW2Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000121
Study TitleImpact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model
Study TypeAnesthesia effect study
Study SummaryWe examined the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia.
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryEvans Lab / Burant Lab
Last NameEvans
First NameCharles
Address6321 Brehm Tower, 1000 Wall Street, Ann Arbor MI 48105
Emailchevans@umich.edu
Phone734-232-8177
Submit Date2014-11-21
Num Groups6
Total Subjects49
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-02-01
Release Version1
Charles Evans Charles Evans
https://dx.doi.org/10.21228/M8GW2Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000108
Project DOI:doi: 10.21228/M8GW2Z
Project Title:Impact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model
Project Type:Anesthesia effect study
Project Summary:We examined, for the first time using untargeted and targeted metabolomics, the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia.
Institute:University of Michigan
Department:Internal Medicine
Laboratory:Evans lab / Burant lab
Last Name:Evans
First Name:Charles
Address:6321 Brehm Tower, 1000 Wall St.
Email:chevans@umich.edu
Phone:734-232-8177
Funding Source:National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health
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