Summary of Study ST000122

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000109. The data can be accessed directly via it's Project DOI: 10.21228/M8C30B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000122
Study TitlePerinatal DDT causes dysfunctional lipid metabolism underlying metabolic syndrome
Study Typesteroid panel
Institute
University of California, Davis
DepartmentNutrition
LaboratoryGaikwad Lab
Last NameGaikwad
First NameNilesh
Emailnwgaikwad@ucdavis.edu
Submit Date2014-09-30
Num GroupsNA
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2014-09-30
Release Version1
Nilesh Gaikwad Nilesh Gaikwad
https://dx.doi.org/10.21228/M8C30B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000109
Project DOI:doi: 10.21228/M8C30B
Project Title:Perinatal DDT causes dysfunctional lipid metabolism underlying metabolic syndrome
Project Type:Pilot and Feasibility Projects
Institute:University of California, Davis
Department:Nutrition
Laboratory:Gaikwad Lab
Last Name:Gaikwad
First Name:Nilesh
Email:nwgaikwad@ucdavis.edu
Phone:530-752-2906
Funding Source:NIH 1U24DK097154 ;  PI Fiehn, Oliver  ; UC Davis WEST COAST CENTRAL COMPREHENSIVE METABOLOMICS RESOURCE CORE (WC3MRC)

Subject:

Subject ID:SU000141
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Tissue/Fluid
SA006749CER346_246320_ML_28Serum
SA006750CER356_269662_ML_29Serum
SA006751CER368_250104_ML_30Serum
SA006752CER369_276355_ML_31Serum
SA006753CER340_244448_ML_27Serum
SA006754CER324_285069_ML_26Serum
SA006755CER287_248530_ML_23Serum
SA006756CER303_253023_ML_24Serum
SA006757CER315_282966_ML_25Serum
SA006758CER384_264971_ML_32Serum
SA006759CER445_286527_ML_33Serum
SA006760CER530_249229_ML_39Serum
SA006761CER540_240346_ML_40Serum
SA006762CER552_241945_ML_41Serum
SA006763CER555_251239_ML_42Serum
SA006764CER488_274343_ML_38Serum
SA006765CER483_294606_ML_37Serum
SA006766CER452_240972_ML_34Serum
SA006767CER463_271249_ML_35Serum
SA006768CER465_265004_ML_36Serum
SA006769CER277_255328_ML_22Serum
SA006770CER226_254303_ML_21Serum
SA006771CER110_238825_ML_7Serum
SA006772CER120_253690_ML_8Serum
SA006773CER147_254803_ML_9Serum
SA006774CER149_266689_ML_10Serum
SA006775CER093_242931_ML_6Serum
SA006776CER085_251176_ML_5Serum
SA006777CER040_242995_ML_2Serum
SA006778CER055_249947_ML_3Serum
SA006779CER062_246153_ML_4Serum
SA006780CER158_254231_ML_11Serum
SA006781CER165_287001_ML_12Serum
SA006782CER216_242490_ML_18Serum
SA006783CER220_274308_ML_19Serum
SA006784CER223_264067_ML_20Serum
SA006785CER201_244193_ML_17Serum
SA006786CER192_254091_ML_16Serum
SA006787CER178_295145_ML_13Serum
SA006788CER181_244392_ML_14Serum
SA006789CER188_250760_ML_15Serum
SA006790CER030_294717_ML_1Serum
Showing results 1 to 42 of 42

Collection:

Collection ID:CO000125
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000143

Sample Preparation:

Sampleprep ID:SP000138
Sampleprep Summary:Methanol: Water Extraction
Sampleprep Protocol Filename:NIH_WCMC_LaMerrill_Method_GaikwadLab__SteroidAnalysis_2013-14.docx
Processing Method:Homogenization and Solvent Removal w/ Speed Vac
Processing Storage Conditions:On Ice
Extraction Method:1:1 Methanol: Water
Extract Concentration Dilution:100mg or 1ml per 4ml 1:1 MeOH:H2O
Extract Storage:-80C
Sample Resuspension:150ul CH3OH/H2O
Organ:Sprague-Dawley Maternal: Adrenal, liver, placenta, amniotic fluid Sprague-Dawley Fetal: Male and female brain, male and female liver

Chromatography:

Chromatography ID:CH000136
Chromatography Summary:Targeted UPLC-MS/MS
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (150 x 2.1mm,1.8um)
Flow Gradient:0-2 min 100% A (Water 0.1% formic acid) 0% B (CH3CN 0.1 % formic acid), 2-4 min 80% A, 4-9mins 45% A, 9-11 mins 20% A, 11-12 mins 100% A
Flow Rate:0.15 ml/min
Sample Injection:10ul
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:12 mins
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN000204
Laboratory Name:Gaikwad Laboratory
Analysis Type:MS
Analysis Comments:UPLC-MS/MS
Acquisition Date:41716
Acquisition Time:12mins
Instrument Name:Waters Acquity UPLC / Xevo-TQ MS
Software Version:Masslynx
Operator Name:Nilesh Gaikwad
Chromatography ID:CH000136
Num Factors:1
Num Metabolites:14
Units:pg/ml
  logo