Summary of Study ST000122

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000109. The data can be accessed directly via it's Project DOI: 10.21228/M8C30B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000122
Study TitlePerinatal DDT causes dysfunctional lipid metabolism underlying metabolic syndrome
Study Typesteroid panel
Institute
University of California, Davis
DepartmentNutrition
LaboratoryGaikwad Lab
Last NameGaikwad
First NameNilesh
Emailnwgaikwad@ucdavis.edu
Submit Date2014-09-30
Num GroupsNA
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2014-09-30
Release Version1
Nilesh Gaikwad Nilesh Gaikwad
https://dx.doi.org/10.21228/M8C30B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000109
Project DOI:doi: 10.21228/M8C30B
Project Title:Perinatal DDT causes dysfunctional lipid metabolism underlying metabolic syndrome
Project Type:Pilot and Feasibility Projects
Institute:University of California, Davis
Department:Nutrition
Laboratory:Gaikwad Lab
Last Name:Gaikwad
First Name:Nilesh
Email:nwgaikwad@ucdavis.edu
Phone:530-752-2906
Funding Source:NIH 1U24DK097154 ;  PI Fiehn, Oliver  ; UC Davis WEST COAST CENTRAL COMPREHENSIVE METABOLOMICS RESOURCE CORE (WC3MRC)

Subject:

Subject ID:SU000141
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Tissue/Fluid
SA006749CER346_246320_ML_28Serum
SA006750CER356_269662_ML_29Serum
SA006751CER368_250104_ML_30Serum
SA006752CER369_276355_ML_31Serum
SA006753CER340_244448_ML_27Serum
SA006754CER324_285069_ML_26Serum
SA006755CER287_248530_ML_23Serum
SA006756CER303_253023_ML_24Serum
SA006757CER315_282966_ML_25Serum
SA006758CER384_264971_ML_32Serum
SA006759CER445_286527_ML_33Serum
SA006760CER530_249229_ML_39Serum
SA006761CER540_240346_ML_40Serum
SA006762CER552_241945_ML_41Serum
SA006763CER555_251239_ML_42Serum
SA006764CER488_274343_ML_38Serum
SA006765CER483_294606_ML_37Serum
SA006766CER452_240972_ML_34Serum
SA006767CER463_271249_ML_35Serum
SA006768CER465_265004_ML_36Serum
SA006769CER277_255328_ML_22Serum
SA006770CER226_254303_ML_21Serum
SA006771CER110_238825_ML_7Serum
SA006772CER120_253690_ML_8Serum
SA006773CER147_254803_ML_9Serum
SA006774CER149_266689_ML_10Serum
SA006775CER093_242931_ML_6Serum
SA006776CER085_251176_ML_5Serum
SA006777CER040_242995_ML_2Serum
SA006778CER055_249947_ML_3Serum
SA006779CER062_246153_ML_4Serum
SA006780CER158_254231_ML_11Serum
SA006781CER165_287001_ML_12Serum
SA006782CER216_242490_ML_18Serum
SA006783CER220_274308_ML_19Serum
SA006784CER223_264067_ML_20Serum
SA006785CER201_244193_ML_17Serum
SA006786CER192_254091_ML_16Serum
SA006787CER178_295145_ML_13Serum
SA006788CER181_244392_ML_14Serum
SA006789CER188_250760_ML_15Serum
SA006790CER030_294717_ML_1Serum
Showing results 1 to 42 of 42

Collection:

Collection ID:CO000125
Collection Summary:-
Sample Type:Blood

Treatment:

Treatment ID:TR000143

Sample Preparation:

Sampleprep ID:SP000138
Sampleprep Summary:Methanol: Water Extraction
Sampleprep Protocol Filename:NIH_WCMC_LaMerrill_Method_GaikwadLab__SteroidAnalysis_2013-14.docx
Processing Method:Homogenization and Solvent Removal w/ Speed Vac
Processing Storage Conditions:On Ice
Extraction Method:1:1 Methanol: Water
Extract Concentration Dilution:100mg or 1ml per 4ml 1:1 MeOH:H2O
Extract Storage:-80C
Sample Resuspension:150ul CH3OH/H2O
Organ:Sprague-Dawley Maternal: Adrenal, liver, placenta, amniotic fluid Sprague-Dawley Fetal: Male and female brain, male and female liver

Combined analysis:

Analysis ID AN000204
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity HSS T3 (150 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Waters Xevo-TQ
Ion Mode POSITIVE
Units pg/ml

Chromatography:

Chromatography ID:CH000136
Chromatography Summary:Targeted UPLC-MS/MS
Instrument Name:Waters Acquity
Column Name:Waters Acquity HSS T3 (150 x 2.1mm,1.8um)
Flow Gradient:0-2 min 100% A (Water 0.1% formic acid) 0% B (CH3CN 0.1 % formic acid), 2-4 min 80% A, 4-9mins 45% A, 9-11 mins 20% A, 11-12 mins 100% A
Flow Rate:0.15 ml/min
Sample Injection:10ul
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:12 mins
Chromatography Type:Reversed phase

MS:

MS ID:MS000167
Analysis ID:AN000204
Instrument Name:Waters Xevo-TQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
Capillary Voltage:3.0 kV
Collision Gas:N2
Ionization:Electrospray Ionization
Source Temperature:150C
Desolvation Gas Flow:600 L/h
Desolvation Temperature:350C
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