Summary of Study ST000135
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000120. The data can be accessed directly via it's Project DOI: 10.21228/M82P4X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000135 |
| Study Title | LCMS analysis of Bile Acids |
| Study Type | drug dosage |
| Study Summary | Mice were dosed with TCDF for 5 days |
| Institute | Pennsylvania State University |
| Department | Molecular Toxicology |
| Last Name | Patterson |
| First Name | Andrew |
| adp117@PSU.EDU, RGN5011@psu.edu | |
| Submit Date | 2015-01-13 |
| Num Groups | 5 |
| Total Subjects | 58 |
| Raw Data Available | No |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-01-13 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000120 |
| Project DOI: | doi: 10.21228/M82P4X |
| Project Title: | Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice |
| Institute: | Pennsylvania State University |
| Department: | Molecular Toxicology |
| Last Name: | Patterson |
| First Name: | Andrew |
| Email: | adp117@PSU.EDU; RGN5011@psu.edu |
Subject:
| Subject ID: | SU000154 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J |
| Age Or Age Range: | 6 weeks old |
| Gender: | Males |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Gender | Genotype |
|---|---|---|---|
| SA007399 | 601 Ileum | Male | Tcdf Treated |
| SA007400 | 404 Feces | Male | Tcdf Treated |
| SA007401 | 402 Ileum | Male | Tcdf Treated |
| SA007402 | 603 Feces | Male | Tcdf Treated |
| SA007403 | 604 Ileum | Male | Tcdf Treated |
| SA007404 | 601 Feces | Male | Tcdf Treated |
| SA007405 | 402 Cecum | Male | Tcdf Treated |
| SA007406 | 604 Cecum | Male | Tcdf Treated |
| SA007407 | 402 Feces | Male | Tcdf Treated |
| SA007408 | 601 Cecum | Male | Tcdf Treated |
| SA007409 | 401 Feces | Male | Tcdf Treated |
| SA007410 | 404 Cecum | Male | Tcdf Treated |
| SA007411 | 603 Cecum | Male | Tcdf Treated |
| SA007412 | 602 Cecum | Male | Tcdf Treated |
| SA007413 | 603 Ileum | Male | Tcdf Treated |
| SA007414 | 404 Ileum | Male | Tcdf Treated |
| SA007415 | 601 Jejunum | Male | Tcdf Treated |
| SA007416 | 404 Liver | Male | Tcdf Treated |
| SA007417 | 602 Jejunum | Male | Tcdf Treated |
| SA007418 | 603 Jejunum | Male | Tcdf Treated |
| SA007419 | 604 Jejunum | Male | Tcdf Treated |
| SA007420 | 603 Liver | Male | Tcdf Treated |
| SA007421 | 601 Liver | Male | Tcdf Treated |
| SA007422 | 602 Liver | Male | Tcdf Treated |
| SA007423 | 602 Ileum | Male | Tcdf Treated |
| SA007424 | 402 Jejunum | Male | Tcdf Treated |
| SA007425 | 404 Jejunum | Male | Tcdf Treated |
| SA007426 | 403 Feces | Male | Tcdf Treated |
| SA007427 | 604 Liver | Male | Tcdf Treated |
| SA007428 | 102 Feces | Male | Wild Type |
| SA007429 | 302 Jejunum | Male | Wild Type |
| SA007430 | 303 Jejunum | Male | Wild Type |
| SA007431 | 304 Jejunum | Male | Wild Type |
| SA007432 | 103 Feces | Male | Wild Type |
| SA007433 | 301 Jejunum | Male | Wild Type |
| SA007434 | 203 Jejunum | Male | Wild Type |
| SA007435 | 202 Jejunum | Male | Wild Type |
| SA007436 | 304 Cecum | Male | Wild Type |
| SA007437 | 304 Ileum | Male | Wild Type |
| SA007438 | 202 Ileum | Male | Wild Type |
| SA007439 | 203 Ileum | Male | Wild Type |
| SA007440 | 302 Ileum | Male | Wild Type |
| SA007441 | 301 Ileum | Male | Wild Type |
| SA007442 | 301 Liver | Male | Wild Type |
| SA007443 | 303 Liver | Male | Wild Type |
| SA007444 | 304 Liver | Male | Wild Type |
| SA007445 | 302 Liver | Male | Wild Type |
| SA007446 | 303 Ileum | Male | Wild Type |
| SA007447 | 301 Cecum | Male | Wild Type |
| SA007448 | 202 Feces | Male | Wild Type |
| SA007449 | 203 Feces | Male | Wild Type |
| SA007450 | 201 Feces | Male | Wild Type |
| SA007451 | 202 Cecum | Male | Wild Type |
| SA007452 | 203 Cecum | Male | Wild Type |
| SA007453 | 302 Cecum | Male | Wild Type |
| SA007454 | 303 Cecum | Male | Wild Type |
| SA007455 | 201 Liver | Male | Wild Type |
| SA007456 | 204 Feces | Male | Wild Type |
| Showing results 1 to 58 of 58 |
Collection:
| Collection ID: | CO000138 |
| Collection Summary: | - |
| Sample Type: | Feces, liver, small and large intestine |
Treatment:
| Treatment ID: | TR000156 |
| Treatment Summary: | Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days |
| Treatment: | Dietary |
| Treatment Compound: | TCDF |
| Treatment Route: | Oral |
| Treatment Dose: | 24 µg/kg body weight |
Sample Preparation:
| Sampleprep ID: | SP000151 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Bile_Acid_Extraction_for_LCMS.docx |
| Processing Method: | Homogenization |
| Processing Storage Conditions: | -20 |
| Extraction Method: | Ethanol extraction |
| Sample Spiking: | Used D4 standards of Cholic Acid , Lithocholic Acid, Ursodeoxycholic Acid, Chenodeoxycholic Acid, and Deoxycholic Acid |
| Organ: | Liver, small and large intestine, and feces |
Chromatography:
| Chromatography ID: | CH000149 |
Analysis:
| Analysis ID: | AN000217 |
| Analysis Type: | MS |
| Instrument Name: | Waters |
| Chromatography ID: | CH000149 |
| Num Factors: | 2 |
| Num Metabolites: | 17 |
| Units: | Peak area |
