Summary of study ST000137

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000122. The data can be accessed directly via it's Project DOI: 10.21228/M8T59G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data
Study IDST000137
Study TitleMetabolomics in sarcoidosis
Study Typeobservation
Study SummaryMetabolites in sarcoidosis patients
Institute
Wayne State University
DepartmentInternal Medicine
Last NameGeamanu
First NameAndreea
Emailageamanu@med.wayne.edu
Submit Date2015-02-11
Total Subjects30
Raw Data AvailableNo
Analysis Type DetailNMR
Release Date2015-02-11
Release Version1
Andreea Geamanu Andreea Geamanu
https://dx.doi.org/10.21228/M8T59G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000122
Project DOI:doi: 10.21228/M8T59G
Project Title:Metabolomics in Sarcoidosis
Institute:Wayne State University
Department:Internal Medicine
Laboratory:Samavati Lab
Last Name:Samavati
First Name:Lobelia
Email:lsamavat@med.wayne.edu

Subject:

Subject ID:SU000156
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Group
SA007469C49Control
SA007470C48Control
SA007471C51Control
SA007472C54Control
SA007473C28Control
SA007474C55Control
SA007475C46Control
SA007476C57Control
SA007477C31Control
SA007478C37Control
SA007479C33Control
SA007480C30Control
SA007481C36Control
SA007482C35Control
SA007483C34Control
SA007484P246Patient
SA007485P233Patient
SA007486P239Patient
SA007487P248Patient
SA007488P232Patient
SA007489P250Patient
SA007490P247Patient
SA007491P197Patient
SA007492P128Patient
SA007493P112Patient
SA007494P138Patient
SA007495P217Patient
SA007496P230Patient
SA007497P218Patient
SA007498P231Patient
Showing results 1 to 30 of 30

Collection:

Collection ID:CO000140
Sample Type:Serum

Treatment:

Treatment ID:TR000158
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000153
Sampleprep Summary:Serum samples were analyzed following filtration through a 3-kD nominal cut-off membrane (Amicon Ultra 0.5 mL centrifugal filters, Millipore Corp, Billerica, MA) to remove proteins, lipids, lipoproteins, and protein-bound forms of certain molecules. Each serum sample was transferred to a prewashed filter and centrifuged at 13,800 g for 60 minutes as previously described (Gregory, Park et al. 2013). The filters were further washed with deuterium oxide (D2O) and the filtrate was mixed with the filtered serum in a 1:1 ratio. A reference buffer solution containing 5 mmol/L disodium-2,2-dimethyl 2-silapentane-5-sulphonate (DSS) and 10 mmol/L imidazole in D2O was added to the samples in a 1:9 ratio. The D2O provided a field-frequency lock, whereas DSS was used as the chemical shift reference and imidazole as pH indicator for the NMR spectra. A total of 600 ┬ÁL mixture was transferred to a 5 mm NMR tube (Sigma Aldrich, St. Louis, MO).

Chromatography:

Chromatography ID:CH000151

Analysis:

Analysis ID:AN000219
Analysis Type:NMR
Chromatography ID:CH000151
Num Factors:2
Num Metabolites:33

NMR:

NMR ID:NM000051
Analysis ID:AN000219
Instrument Name:Agilent
Instrument Type:FT-NMR
NMR Experiment Type:NOESY
Spectrometer Frequency:600 MHz
  logo