Summary of Study ST000142
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000127. The data can be accessed directly via it's Project DOI: 10.21228/M8959T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000142 |
| Study Title | H1299 13C-labeled Cell Study |
| Study Type | tracer |
| Study Summary | [U-13C]-Glu SIRM study of H1299 cells with QDA treatment |
| Institute | University of Kentucky |
| Department | CESB |
| Last Name | Fan |
| First Name | Teresa |
| Address | Rm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536 |
| twmfan@gmail.com | |
| Submit Date | 2015-03-12 |
| Raw Data Available | No |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-03-12 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000127 |
| Project DOI: | doi: 10.21228/M8959T |
| Project Title: | Evaluation of QDA chemoselection |
| Project Summary: | Demonstrate the effectiveness of QDA/13CD3-QDA chemoselection experimental protocol on stable isotope tracer based metabolomic investigation of cell metabolism |
| Institute: | University of Kentucky |
| Department: | CESB |
| Last Name: | Fan |
| First Name: | Teresa |
| Address: | Rm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536 |
| Email: | twmfan@gmail.com |
Subject:
| Subject ID: | SU000161 |
| Subject Type: | Human cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | H1299 |
| Species Group: | Mammals |
Factors:
Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA007970 | H1299_Ctl_3_13C6Glc_plate | 13C-Glc |
| SA007971 | H1299_Ctl_2_13C6Glc_plate | 13C-Glc |
| SA007972 | H1299_Ctl_1_13C6Glc_plate | 13C-Glc |
| SA007973 | H1299_Ctl_1_unL_plate | unlabeled |
| Showing results 1 to 4 of 4 |
Collection:
| Collection ID: | CO000146 |
| Collection Summary: | - |
| Collection Protocol Filename: | WS_Fan_Quench_Cell_Tissue.pdf |
| Collection Protocol Comments: | Collection and quench of cells in tissue culture |
| Sample Type: | Lung |
Treatment:
| Treatment ID: | TR000164 |
| Treatment Protocol Filename: | WS_Fan_Cell_Tracer_Ex.pdf |
| Treatment Protocol Comments: | cell culture in 13C-Glc-labeled media for 24h with QDA derivatives |
Sample Preparation:
| Sampleprep ID: | SP000159 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | WS_Fan_Extract_Polar_Lipid_Prot.pdf |
| Sampleprep Protocol Comments: | Polar extraction from cells. |
Chromatography:
| Chromatography ID: | CH000156 |
Analysis:
| Analysis ID: | AN000225 |
| Laboratory Name: | RCSIRM |
| Analysis Type: | MS |
| Analysis Comments: | FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formulas. |
| Instrument Name: | Bruker Solarix |
| Chromatography ID: | CH000156 |
| Num Factors: | 2 |
| Num Metabolites: | 2846 |
| Units: | Peak area |
