Summary of study ST000144

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000129. The data can be accessed directly via it's Project DOI: 10.21228/M85C7W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000144
Study TitlePrimary T Cell Baseline (Donor 5) - II
Study TypeCell labeling
Study SummaryLabeling of cells was carried out in triplicate with each unstimulated sample containing 20e6 cells and stimulated cells containing 12.8e6 cells. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled medium. At this time cells were also activated with 1 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 12 h when 1 ml of spent medium was collected and cells were resuspended in 200 ul ice cold methanol. All samples were immediately stored in -80°C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-03-13
Num Groups4
Total Subjects27
Raw Data AvailableYes
Raw Data File Type(s).cd,.cG, .xml, .bin,.stg
Uploaded File Size750 M
Analysis Type DetailGC/LC-MS
Release Date2015-03-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M85C7W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000129
Project DOI:doi: 10.21228/M85C7W
Project Title:Metabolic effects of Noxa
Project Summary:labeled glutamine metabolomics on Noxa expressing cells
Institute:University of Minnesota
Department:Masonic Cancer Center
Laboratory:Kelekar Lab
Last Name:Kelekar
First Name:Ameeta
Address:420 Delaware Street SE, Minneapolis, MN 55455
Email:ameeta@umn.edu
Phone:612-626-2358

Subject:

Subject ID:SU000163
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Label Stimulated Donor
SA008004S00015405Glucose N 5
SA008005S00015406Glucose N 5
SA008006S00015407Glucose N 5
SA008007S00015393Glucose N 5
SA008008S00015394Glucose N 5
SA008009S00015395Glucose N 5
SA008010S00015408Glucose Y 5
SA008011S00015409Glucose Y 5
SA008012S00015410Glucose Y 5
SA008013S00015398Glucose Y 5
SA008014S00015396Glucose Y 5
SA008015S00015397Glucose Y 5
SA008016S00015432Glutamine N 6
SA008017S00015434Glutamine N 6
SA008018S00015433Glutamine N 6
SA008019S00015422Glutamine N 6
SA008020S00015420Glutamine N 6
SA008021S00015421Glutamine N 6
SA008022S00015435Glutamine Y 6
SA008023S00015436Glutamine Y 6
SA008024S00015437Glutamine Y 6
SA008025S00015424Glutamine Y 6
SA008026S00015423Glutamine Y 6
SA008027S00015425Glutamine Y 6
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000149
Sample Type:Cells

Treatment:

Treatment ID:TR000168

Sample Preparation:

Sampleprep ID:SP000163
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.pdf

Combined analysis:

Analysis ID AN000228 AN000229
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area

Chromatography:

Chromatography ID:CH000158
Methods ID:AQM010
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001299
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000179
Analysis ID:AN000228
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000180
Analysis ID:AN000229
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
Tube Lens Voltage:Positive
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