Summary of study ST000148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000127. The data can be accessed directly via it's Project DOI: 10.21228/M8959T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000148
Study TitleA549 13C-labeled Cell Study
Study Typetracer
Study Summary[U-13C]-Glu SIRM study of A549 cells with QDA treatment
Institute
University of Kentucky
DepartmentCESB
Last NameFan
First NameTeresa
AddressRm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536
Emailtwmfan@gmail.com
Submit Date2015-03-24
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-03-24
Release Version1
Teresa Fan Teresa Fan
https://dx.doi.org/10.21228/M8959T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000127
Project DOI:doi: 10.21228/M8959T
Project Title:Evaluation of QDA chemoselection
Project Summary:Demonstrate the effectiveness of QDA/13CD3-QDA chemoselection experimental protocol on stable isotope tracer based metabolomic investigation of cell metabolism
Institute:University of Kentucky
Department:CESB
Last Name:Fan
First Name:Teresa
Address:Rm 516 Biopharm Complex, 789 S. Limestone St.,Univ. of Kentucky, Lexington, KY 40536
Email:twmfan@gmail.com

Subject:

Subject ID:SU000167
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:A549
Species Group:Human

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA008085A549_Ctl_3_13C6Glc_plate13C-Glc
SA008086A549_Ctl_1_13C6Glc_plate13C-Glc
SA008087A549_Ctl_2_13C6Glc_plate13C-Glc
SA008088A549_Ctl_1_unL_plateunlabeled
SA008089A549_Ctl_2_unL_plateunlabeled
Showing results 1 to 5 of 5

Collection:

Collection ID:CO000153
Collection Protocol Filename:WS_Fan_Quench_Cell_Tissue.pdf
Collection Protocol Comments:Collection and quench of cells in tissue culture
Sample Type:Epithelial cells

Treatment:

Treatment ID:TR000172
Treatment Summary:unlabeled | 13C-Glc
Treatment Protocol Filename:WS_Fan_Cell_Tracer_Ex.pdf
Treatment Protocol Comments:cell culture in unlabeled media for 24h with QDA derivatives | cell culture in 13C-Glc-labeled media for 24h with QDA derivatives

Sample Preparation:

Sampleprep ID:SP000167
Sampleprep Summary:polar extraction | protein extraction | lipid extraction
Sampleprep Protocol Filename:WS_Fan_Extract_Polar_Lipid_Prot.pdf_
_WS_Fan_Prot_Quant.pdf_
_WS_Fan_Extract_Polar_Lipid_Prot.pdf
Sampleprep Protocol Comments:Polar extraction from cells | Protein extraction and quantification | Lipid extraction from cells

Combined analysis:

Analysis ID AN000235
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type ESI
MS instrument type FT-ICR-MS
MS instrument name Bruker SolariX FT-ICR-MS
Ion Mode POSITIVE
Units normalized corrected Peak area

Chromatography:

Chromatography ID:CH000164

MS:

MS ID:MS000186
Analysis ID:AN000235
Instrument Name:Bruker SolariX FT-ICR-MS
Instrument Type:FT-ICR-MS
MS Type:ESI
Ion Mode:POSITIVE
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