Summary of study ST000176

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000148. The data can be accessed directly via it's Project DOI: 10.21228/M8V011 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000176
Study Titlecell and liver metabolomics
Study Summary(Pending) Liver samples were harvested from wt and ko mice. Quadruplicates and triplicates are sent for cells and liver, respectively.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2014-05-12
Num Groups4
Total Subjects14
Raw Data AvailableYes
Raw Data File Type(s).xml,.xsd,.stg, .bin, .cd,.cG, .m
Uploaded File Size3.1 G
Analysis Type DetailGC/LC-MS
Release Date2015-06-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8V011
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000148
Project DOI:doi: 10.21228/M8V011
Project Title:trcp
Project Summary:metabolic phenotypes of a clock mutant mouse
Institute:Florida State University
Department:Biomedical Sciences
Laboratory:Lee Lab
Last Name:Lee
First Name:Choogon
Address:Tallahassee, FL
Email:choogon.lee@med.fsu.edu
Phone:850-645-1478

Subject:

Subject ID:SU000195
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id genotype Type
SA009475S00014601background liver
SA009476S00014600background liver
SA009477S00014602background liver
SA009471S00014608background MFE Cells
SA009472S00014609background MFE Cells
SA009473S00014607background MFE Cells
SA009474S00014606background MFE Cells
SA009482S00014604knock-out liver
SA009483S00014605knock-out liver
SA009484S00014603knock-out liver
SA009478S00014612knock-out MFE Cells
SA009479S00014613knock-out MFE Cells
SA009480S00014611knock-out MFE Cells
SA009481S00014610knock-out MFE Cells
Showing results 1 to 14 of 14

Collection:

Collection ID:CO000182
Sample Type:Cells

Treatment:

Treatment ID:TR000202

Sample Preparation:

Sampleprep ID:SP000196
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000270 AN000271
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6530 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units pmol/mg protein SUC

Chromatography:

Chromatography ID:CH000193
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001303
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000219
Analysis ID:AN000270
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000220
Analysis ID:AN000271
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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