Summary of Study ST000180
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000155. The data can be accessed directly via it's Project DOI: 10.21228/M82G6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000180 |
| Study Title | 2 lines of pulmonary artery smooth muscle cells metabolic profile (122 vs 3633C central metabolic profile) |
| Study Type | Glycolysis/TCA/Nucleotide analysis (tissue/cells) |
| Study Summary | 2 lines of pulmonary artery smooth muscle cells which has been snap frozen during active growth phase |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-06 |
| Num Groups | 2 |
| Total Subjects | 10 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 1.5 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-06-18 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000155 |
| Project DOI: | doi: 10.21228/M82G6M |
| Project Title: | PASMC metabolism |
| Project Summary: | Comparison of metabolic attributes of vascular smooth muscle cells |
| Institute: | University of California, San Francisco |
| Department: | Pediatrics |
| Laboratory: | Maltepe Lab |
| Last Name: | Maltepe |
| First Name: | Emin |
| Address: | San Francisco, CA |
| Email: | Emin.Maltepe@ucsf.edu |
| Phone: | 415-476-9313 |
Subject:
| Subject ID: | SU000199 |
| Subject Type: | Animal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Ovis aries (Factor headings shown in green)
| mb_sample_id | local_sample_id | Animal |
|---|---|---|
| SA009507 | S00015063 | control |
| SA009508 | S00015064 | control |
| SA009509 | S00015065 | control |
| SA009510 | S00015066 | control |
| SA009511 | S00015062 | control |
| SA009512 | S00015061 | shunt |
| SA009513 | S00015058 | shunt |
| SA009514 | S00015059 | shunt |
| SA009515 | S00015060 | shunt |
| SA009516 | S00015057 | shunt |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000186 |
| Collection Summary: | - |
| Sample Type: | Cells |
Treatment:
| Treatment ID: | TR000206 |
Sample Preparation:
| Sampleprep ID: | SP000200 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx |
Chromatography:
| Chromatography ID: | CH000200 |
| Methods ID: | AQM020 |
| Methods Filename: | QTOF-002-HILIC-35min-1mm.m.zip |
| Instrument Name: | Agilent 1260 |
| Column Name: | Phenomenex Luna NH2 (150 x 1mm,3um) |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN000278 |
| Laboratory Name: | MRC2 (University of Michigan) |
| Analysis Type: | MS |
| Acquisition ID: | AQM020 |
| Acquisition Parameters File: | Column1_solv1_jetstream+_grad9.m.zip |
| Instrument Name: | Agilent 6530 QTOF |
| Processing Parameters File: | EX00314-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip |
| Chromatography ID: | CH000200 |
| Num Factors: | 2 |
| Num Metabolites: | 25 |
| Units: | pmol/µg protein |
