Summary of Study ST000180

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000155. The data can be accessed directly via it's Project DOI: 10.21228/M82G6M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000180
Study Title2 lines of pulmonary artery smooth muscle cells metabolic profile (122 vs 3633C central metabolic profile)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study Summary2 lines of pulmonary artery smooth muscle cells which has been snap frozen during active growth phase
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-06
Num Groups2
Total Subjects10
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size1.5 G
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M82G6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000155
Project DOI:doi: 10.21228/M82G6M
Project Title:PASMC metabolism
Project Summary:Comparison of metabolic attributes of vascular smooth muscle cells
Institute:University of California, San Francisco
Department:Pediatrics
Laboratory:Maltepe Lab
Last Name:Maltepe
First Name:Emin
Address:San Francisco, CA
Email:Emin.Maltepe@ucsf.edu
Phone:415-476-9313

Subject:

Subject ID:SU000199
Subject Type:Animal
Subject Species:Ovis aries
Taxonomy ID:9940
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Ovis aries (Factor headings shown in green)

mb_sample_id local_sample_id Animal
SA009507S00015063control
SA009508S00015064control
SA009509S00015065control
SA009510S00015066control
SA009511S00015062control
SA009512S00015061shunt
SA009513S00015058shunt
SA009514S00015059shunt
SA009515S00015060shunt
SA009516S00015057shunt
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000186
Collection Summary:-
Sample Type:Cells

Treatment:

Treatment ID:TR000206

Sample Preparation:

Sampleprep ID:SP000200
Sampleprep Summary:-
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000278
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode NEGATIVE
Units pmol/µg protein

Chromatography:

Chromatography ID:CH000200
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Chromatography Type:HILIC

MS:

MS ID:MS000227
Analysis ID:AN000278
Instrument Name:Agilent 6530 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
Acquisition Parameters File:Column1_solv1_jetstream+_grad9.m.zip
Processing Parameters File:EX00314-MassHunterQuant-GlyTCA-DataAnalysis-LCMS-Method.m.zip
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