Summary of study ST000181

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000153. The data can be accessed directly via it's Project DOI: 10.21228/M8B01C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000181
Study TitleT cell metabolism during graft-versus-host disease(CAB 311)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummaryT cells were injected into mice to model graft-versus-host disease. On day 7 after bone marrow transplantation (BMT), cells were recovered and CD8 T cells were purified to > 90% purity over magnetic columns. Naïve T cells were used as control. Cells were then plated at 5x10^6 cells/ml onto plates coated with anti-CD3/CD28 antibodies in the presence of 300µM 13C-palmitate conjugated to BSA (in PBS). Cells were incubated at 37oC for 60min, after which time they were removed from the plates, counted, and an equal number (2.5x10^6) were placed into a 1.7 ml micro-centrifuge tubes and spun down. Cells were washed once with ammonium chloride, residual volume was removed by a second brief spin, cell pellets were flash frozen in liquid nitrogen and stored at -80oC until analysis.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-07
Num Groups2
Total Subjects8
Raw Data AvailableYes
Raw Data File Type(s).xml,.xsd,.stg, .bin, .cd,.cG, .m (lcms)
Uploaded File Size1.2 G
Analysis Type DetailLC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8B01C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000153
Project DOI:doi: 10.21228/M8B01C
Project Title:Graft-Versus-Host Disease
Project Summary:T cell metabolism during graft-versus-host disease
Institute:University of Michigan
Department:Pediatrics
Laboratory:Ferrara lab
Last Name:Byersdorfer
First Name:Craig
Address:Ann Arbor, MI
Email:craigbye@med.umich.edu
Phone:734-000-0000
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