Summary of study ST000194

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000165. The data can be accessed directly via it's Project DOI: 10.21228/M8WS31 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data (Contains raw data)
Study IDST000194
Study TitleLanthanide-mineral induced alteration of bile acid metabolism in a murine model of steatohepatitis
Study Summary120 mice (equal number of males and females) were randomized into 3 equal groups. One group is on a HFWD alone, a second group is on HFWD supplemented with lanthanides and calcium, and a third control group is supplemented with calcium alone. At study termination (18 months) we will harvest hepatocytes and colonic enterocytes for evaluation of epithelial gene expression patterns. We will also harvest cecal contents and feces for microbial profiling by 16S rRNA pyrosequencing. For this small pilot proposal, we wish to add an untargeted metabolomic analysis component. We will harvest liver (right medial lobe with gall bladder), serum, and feces. We will assay representative liver/gall bladder samples (8 from the HFWD group and 8 from the lanthanide/calcium supplemented group). Remaining liver samples and the serum and feces will be archived for future investigation. Liver is being targeted first since both steatohepatitis and hepatocellular carcinoma were seen in our previous study in mice on HFWD [2]. Additionally, alterations of bile acid profiles and bile acid metabolism have been associated with both steatohepatitis and hepatic cancers.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-09
Num Groups20
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s).xml,.xsd,.stg, .bin, .cd,.cG
Uploaded File Size64 M
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8WS31
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000165
Project DOI:doi: 10.21228/M8WS31
Project Title:Tumor Suppression
Project Summary:Tumor suppression by diet in mice fed HFWD
Institute:University of Michigan
Department:Animal Medicine
Laboratory:Bergin Lab
Last Name:Bergin
First Name:Ingrid
Address:Ann Arbor, MI
Email:ingridbe@med.umich.edu
Phone:734-936-1701

Subject:

Subject ID:SU000213
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet
SA009775S00015181HFWD
SA009776S00015182HFWD
SA009777S00015183HFWD
SA009778S00015184HFWD
SA009779S00015175HFWD
SA009780S00015176HFWD
SA009781S00015177HFWD
SA009782S00015178HFWD
SA009783S00015179HFWD
SA009784S00015180HFWD
SA009766S00015185HFWD + Calcium
SA009767S00015186HFWD + Calcium
SA009768S00015191HFWD + Calcium
SA009769S00015187HFWD + Calcium
SA009770S00015193HFWD + Calcium
SA009771S00015192HFWD + Calcium
SA009772S00015188HFWD + Calcium
SA009773S00015189HFWD + Calcium
SA009774S00015190HFWD + Calcium
Showing results 1 to 19 of 19

Collection:

Collection ID:CO000201
Sample Type:Liver

Treatment:

Treatment ID:TR000221

Sample Preparation:

Sampleprep ID:SP000215
Sampleprep Protocol Filename:Bile-acids-2015-03-11.docx

Combined analysis:

Analysis ID AN000296
Analysis type MS
Chromatography type Reversed phase
Chromatography system
Column Waters Acquity BEH C18 (50 x 2.1mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode NEGATIVE
Units pmol/mg sample

Chromatography:

Chromatography ID:CH000217
Methods ID:AQM041
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-h.m.zip
Column Name:Waters Acquity BEH C18 (50 x 2.1mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000245
Analysis ID:AN000296
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
  logo