Summary of study ST000213

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000171. The data can be accessed directly via it's Project DOI: 10.21228/M8HG6N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000213
Study TitleGermfree vs Conventional Swiss webster mice Studies (part II)
Study TypeBile acid analysis (colon contents/plasma)
Study SummaryThe intestinal tract is inhabited by a large and diverse community of microbes collectively referred to as the gut microbiota. While the gut microbiota provides important benefits to its host, especially in metabolism and immune development, disturbance of the microbiota–host relationship is associated with numerous chronic inflammatory diseases, including inflammatory bowel disease and the group of obesity-associated diseases collectively referred to as metabolic syndrome. A primary means by which the intestine is protected from its microbiota is via multi-layered mucus structures that cover the intestinal surface, thereby allowing the vast majority of gut bacteria to be kept at a safe distance from epithelial cells that line the intestine1. Thus, agents that disrupt mucus–bacterial interactions might have the potential to promote diseases associated with gut inflammation. Consequently, it has been hypothesized that emulsifiers, detergent-like molecules that are a ubiquitous component of processed foods and that can increase bacterial translocation across epithelia in vitro2, might be promoting the increase in inflammatory bowel disease observed since the mid-twentieth century3. Here we report that, in mice, relatively low concentrations of two commonly used emulsifiers, namely carboxymethylcellulose and polysorbate-80, induced low-grade inflammation and obesity/metabolic syndrome in wild-type hosts and promoted robust colitis in mice predisposed to this disorder. Emulsifier-induced metabolic syndrome was associated with microbiota encroachment, altered species composition and increased pro-inflammatory potential. Use of germ-free mice and faecal transplants indicated that such changes in microbiota were necessary and sufficient for both low-grade inflammation and metabolic syndrome. These results support the emerging concept that perturbed host–microbiota interactions resulting in low-grade inflammation can promote adiposity and its associated metabolic effects. Moreover, they suggest that the broad use of emulsifying agents might be contributing to an increased societal incidence of obesity/metabolic syndrome and other chronic inflammatory diseases. In metadata factor “Treatment” CMC stands for carboxymethylcellulose and P80 for polysorbate-80 Research is published: http://www.nature.com/nature/journal/v519/n7541/full/nature14232.html
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-12
Num Groups6
Total Subjects66
Raw Data AvailableYes
Raw Data File Type(s).xml,.xsd,.stg, .bin, .cd,.cG
Uploaded File Size64 M
Analysis Type DetailLC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8HG6N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000171
Project DOI:doi: 10.21228/M8HG6N
Project Title:Dietary emulsifying agents
Project Summary:SCFA and Bile acid composition in conventional and GermFree SW treated with emulsifiers
Institute:Georgia State University
Department:Biology
Laboratory:Gewirtz's Lab
Last Name:Gewirtz
First Name:Andrew
Address:14 Marietta Street, NW Atlanta, GA 30303-2813
Email:bchassaing@gsu.edu
Phone:404-413-3589

Subject:

Subject ID:SU000232
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id treatment Germfree versus conventional
SA010489S00012779CMC Conventionaly raised animal
SA010490S00012780CMC Conventionaly raised animal
SA010491S00012778CMC Conventionaly raised animal
SA010492S00012813CMC Conventionaly raised animal
SA010493S00012814CMC Conventionaly raised animal
SA010494S00012781CMC Conventionaly raised animal
SA010495S00012782CMC Conventionaly raised animal
SA010496S00012812CMC Conventionaly raised animal
SA010497S00012811CMC Conventionaly raised animal
SA010498S00012785CMC Conventionaly raised animal
SA010499S00012784CMC Conventionaly raised animal
SA010500S00012783CMC Conventionaly raised animal
SA010501S00012816CMC Conventionaly raised animal
SA010502S00012815CMC Conventionaly raised animal
SA010503S00012817CMC Conventionaly raised animal
SA010504S00012818CMC Conventionaly raised animal
SA010505S00012765CMC Germfree animal
SA010506S00012764CMC Germfree animal
SA010507S00012799CMC Germfree animal
SA010508S00012798CMC Germfree animal
SA010509S00012800CMC Germfree animal
SA010510S00012766CMC Germfree animal
SA010511S00012767CMC Germfree animal
SA010512S00012797CMC Germfree animal
SA010513S00012820P80 Conventionaly raised animal
SA010514S00012819P80 Conventionaly raised animal
SA010515S00012825P80 Conventionaly raised animal
SA010516S00012824P80 Conventionaly raised animal
SA010517S00012822P80 Conventionaly raised animal
SA010518S00012821P80 Conventionaly raised animal
SA010519S00012823P80 Conventionaly raised animal
SA010520S00012792P80 Conventionaly raised animal
SA010521S00012788P80 Conventionaly raised animal
SA010522S00012791P80 Conventionaly raised animal
SA010523S00012789P80 Conventionaly raised animal
SA010524S00012787P80 Conventionaly raised animal
SA010525S00012786P80 Conventionaly raised animal
SA010526S00012790P80 Conventionaly raised animal
SA010527S00012804P80 Germfree animal
SA010528S00012805P80 Germfree animal
SA010529S00012772P80 Germfree animal
SA010530S00012803P80 Germfree animal
SA010531S00012802P80 Germfree animal
SA010532S00012768P80 Germfree animal
SA010533S00012769P80 Germfree animal
SA010534S00012770P80 Germfree animal
SA010535S00012771P80 Germfree animal
SA010536S00012801P80 Germfree animal
SA010537S00012776Water Conventionaly raised animal
SA010538S00012773Water Conventionaly raised animal
SA010539S00012777Water Conventionaly raised animal
SA010540S00012774Water Conventionaly raised animal
SA010541S00012775Water Conventionaly raised animal
SA010542S00012808Water Conventionaly raised animal
SA010543S00012810Water Conventionaly raised animal
SA010544S00012806Water Conventionaly raised animal
SA010545S00012807Water Conventionaly raised animal
SA010546S00012809Water Conventionaly raised animal
SA010547S00012763Water Germfree animal
SA010548S00012762Water Germfree animal
SA010549S00012793Water Germfree animal
SA010550S00012761Water Germfree animal
SA010551S00012796Water Germfree animal
SA010552S00012760Water Germfree animal
SA010553S00012795Water Germfree animal
SA010554S00012794Water Germfree animal
Showing results 1 to 66 of 66

Collection:

Collection ID:CO000220
Sample Type:Faces

Treatment:

Treatment ID:TR000240
Treatment Summary:In metadata factor “Treatment” CMC stands for carboxymethylcellulose and P80 for polysorbate-80

Sample Preparation:

Sampleprep ID:SP000234
Sampleprep Protocol Filename:Bile-acids-2015-03-11.docx

Combined analysis:

Analysis ID AN000315
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode NEGATIVE
Units pmol/mg sample

Chromatography:

Chromatography ID:CH000236
Methods ID:AQM040
Methods Filename:2014-03-27-Bile_acid-full-ESN-insert.m.zip

MS:

MS ID:MS000264
Analysis ID:AN000315
Instrument Name:Agilent 6490 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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