Summary of study ST000215

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000169. The data can be accessed directly via it's Project DOI: 10.21228/M8N88K This work is supported by NIH grant, U2C- DK119886.

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Study IDST000215
Study TitleLiver and Plasma metaboites for 13C-glucose load in wild type, LIRKO GTT 1 mice
Study Type13C mass isotopomer analysis (flux studies)
Study SummaryMice were fasted for 18 hr overnight then sacrificed or treated with 13C-U-glucose (2 g/kg ip) and sacrificed 1 hr later by decapitation and liver was immediately freeze-clamped and stored in liquid N2 and then at -80 C. Wild type (IR and IR/FoxO1 floxed) mice were sacrificed after fasting and 1 hr post-glucose treatment. Liver-specific insulin receptor knockout (LIRKO) and insulin receptor/FoxO1 double knockout (LIRFKO) mice were sacrificed 1 hr post glucose treatment. http://www.nature.com/ncomms/2015/150512/ncomms8079/full/ncomms8079.html
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-06-12
Num Groups5
Total Subjects40
Raw Data AvailableYes
Raw Data File Type(s).xml,.xsd,.stg, .bin, .cd,.cG
Uploaded File Size2.5 G
Analysis Type DetailGC/LC-MS
Release Date2015-12-28
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8N88K
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000169
Project DOI:doi: 10.21228/M8N88K
Project Title:Regulation of hepatic metabolism
Project Summary:Examine metabolite in liver and plasma before and after 13C-glucose load in wild type, LIRKO and LIRFKO mice
Institute:University of Chicago
Department:Jesse Brown VA Medical Center
Laboratory:Unterman Lab
Last Name:Unterman
First Name:Terry
Address:Chicago, IL
Email:unterman@uic.edu
Phone:847-778-0943

Subject:

Subject ID:SU000234
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id treatment Strain
SA010568S00013145glucose IRF floxed
SA010565S00013143glucose IR floxed
SA010566S00013144glucose IR floxed
SA010567S00013142glucose IR floxed
SA010569S00013152glucose LIRFKO
SA010570S00013154glucose LIRFKO
SA010571S00013153glucose LIRFKO
SA010572S00013155glucose LIRFKO
SA010573S00013149glucose LIRKO
SA010574S00013150glucose LIRKO
SA010575S00013151glucose LIRKO
SA010576S00013148glucose LIRKO
SA010577S00013147glucose LIRKO
SA010578S00013146glucose LIRKO
SA010579S00013139none IR floxed
SA010580S00013141none IR floxed
SA010581S00013138none IR floxed
SA010582S00013137none IR floxed
SA010583S00013136none IR floxed
SA010584S00013140none IR floxed
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000222
Sample Type:Liver

Treatment:

Treatment ID:TR000242

Sample Preparation:

Sampleprep ID:SP000236
Sampleprep Protocol Filename:Fluxomics_analysis_protocol-2015-03-11.docx

Combined analysis:

Analysis ID AN000317 AN000318
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units Area Area

Chromatography:

Chromatography ID:CH000238
Methods ID:AQM010
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001306
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC

MS:

MS ID:MS000266
Analysis ID:AN000317
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
  
MS ID:MS000267
Analysis ID:AN000318
Instrument Name:Agilent 5975C
Instrument Type:Single quadrupole
MS Type:EI
Ion Mode:POSITIVE
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