Summary of Study ST000225
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000120. The data can be accessed directly via it's Project DOI: 10.21228/M82P4X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000225 |
| Study Title | Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice |
| Study Type | drug dosage |
| Study Summary | Mice were dosed with TCDF for 5 days |
| Institute | Pennsylvania State University |
| Department | Molecular Toxicology |
| Last Name | Patterson |
| First Name | Andrew |
| adp117@PSU.EDU | |
| Submit Date | 2015-06-08 |
| Raw Data Available | No |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2015-06-08 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000120 |
| Project DOI: | doi: 10.21228/M82P4X |
| Project Title: | Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice |
| Institute: | Pennsylvania State University |
| Department: | Molecular Toxicology |
| Last Name: | Patterson |
| First Name: | Andrew |
| Email: | adp117@PSU.EDU; RGN5011@psu.edu |
Subject:
| Subject ID: | SU000244 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6J; Wild Type and Ahr-/- |
| Age Or Age Range: | 6 weeks old |
| Gender: | Males |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Gender | Genotype |
|---|---|---|---|
| SA010796 | Control Liver | Male | Control |
| SA010797 | Control fecal | Male | Control |
| SA010798 | Control cecal content | Male | Control |
| SA010799 | Treated Cecum | Male | Tcdf Treated |
| SA010800 | Treated Ileum | Male | Tcdf Treated |
| SA010801 | Treated Jejunum | Male | Tcdf Treated |
| SA010802 | Treated Fecal | Male | Tcdf Treated |
| SA010803 | Treated Cecal Content | Male | Tcdf Treated |
| SA010804 | Treated Duodenum | Male | Tcdf Treated |
| SA010805 | Treated Liver | Male | Tcdf Treated |
| Showing results 1 to 10 of 10 |
Collection:
| Collection ID: | CO000232 |
| Collection Summary: | - |
| Sample Type: | Feces, liver, cecal contents, and intestine |
Treatment:
| Treatment ID: | TR000252 |
| Treatment Summary: | Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days |
| Treatment: | Dietary |
| Treatment Compound: | TCDF |
| Treatment Route: | Oral |
| Treatment Dose: | 24 µg/kg body weight |
Sample Preparation:
| Sampleprep ID: | SP000246 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | NMR_metabolomics_protocol.docx |
| Processing Method: | Homogenization |
| Processing Storage Conditions: | -20 |
| Extraction Method: | Methanol/Water |
| Extract Concentration Dilution: | 2 Methanol : 1 Water |
| Sample Resuspension: | D2O |
| Sample Spiking: | TSP-d4 |
| Organ: | Liver, small and large intestine |
Analysis:
| Analysis ID: | AN000335 |
| Analysis Type: | NMR |
| Chromatography ID: | CH000251 |
| Num Factors: | 2 |
NMR:
| NMR ID: | NM000054 |
| Analysis ID: | AN000335 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | NOESY PR1D |
| Spectrometer Frequency: | 600 MHz |
| NMR Solvent: | D2O |
