Summary of Study ST000235

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000188. The data can be accessed directly via it's Project DOI: 10.21228/M8ZK5B This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000235
Study TitleSprague Dawley rats Nicotine alters brain oxidative metabolism
Study SummaryAdult (14 weeks old) Sprague-Dawley rats showing at least three consecutive normal periods (4 day) of estrous cycles were randomly assigned to four groups: 1: saline, 2: nicotine (6 mg/kg), 3: OC and 4: nicotine (6 mg/kg) + OC. Rats were exposed to these treatments for a month. At the end of treatments, hippocampus was obtained, immediately frozen in liquid nitrogen. We are sending one side of hippocampi to Southeast Center for Integrated Metabolomics for analysis at the University of Florida.
Institute
University of Florida
DepartmentSECIM
Last NameGarrett
First NameTim
Address2004 Mowry Road
Emailtgarrett@ufl.edu
Phone3526279177
Submit Date2015-08-25
Total Subjects32
Study CommentsSaline, Nicotine, Oral contraceptives, Nicotine + oral contraceptives
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Uploaded File Size4.2 G
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Tim Garrett Tim Garrett
https://dx.doi.org/10.21228/M8ZK5B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000188
Project DOI:doi: 10.21228/M8ZK5B
Project Title:Nicotine alters brain oxidative metabolism
Project Type:Pilot
Project Summary:Nicotine addiction through the use of cigarettes is the most common form of chemical dependency in the United States. Despite global warnings and awareness of the detrimental effects of smoking on health, smoking-derived nicotine dependence makes it very difficult to relinquish the habit. Unfortunately, nicotine exposure makes females more susceptible to ischemic neurodegeneration. Moreover, this susceptibility is enhanced when combined with oral contraceptives. Usually, women initiate nicotine usage during their adolescence, the period of life when one also is likely to get sexually active and also likely to use oral contraceptives. The underlying mechanisms responsible for the exacerbated neurodegeneration due to the long exposure of nicotine and oral contraceptives are not known. Our published studies showed that nicotine toxicity is exacerbated by oral contraceptives via altered mitochondrial function and increased reactive oxygen species production in the hippocampus of female rats. This mitochondrial dysfunction involved a defect on the enzymatic activity of the terminal enzyme of the electron transport chain (complex IV) as a consequence of an impaired biogenesis. However, how this mitochondrial impairment impacts the overall brain metabolism remains to be investigated. To understand the overall effect of nicotine on the brain metabolism and the mechanisms responsible for the enhanced toxicity of nicotine when combined with oral contraceptives, we propose two specific aims. We will investigate the metabolomic profile of brains (hippocampus) of female rats treated with nicotine alone or in combination with oral contraceptives. We will also determine the effect on the activity and steady-state levels of key regulatory metabolic enzymes. We will use an established rat animal model mimicking conditions of nicotine exposure produced by cigarette smoking and simulating conditions of oral contraceptives usage taking in consideration hormonal cycles in women. Discerning the exact effects of nicotine and its combination with oral contraceptives on overall brain metabolisms at different ages will open a new window for future therapeutic intervention. This intervention will reduce their susceptibility to neurodegenerative conditions in women trying to give up nicotine addiction.
Institute:University of Miami
Department:Neurology
Laboratory:CVDRC
Last Name:Raval
First Name:Ami
Address:1420 Nw, 9th Ave, Miami, FL -33136
Email:araval@med.miami.edu
Phone:305-243-7491
Funding Source:AHA

Subject:

Subject ID:SU000254
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Genotype Strain:Sprague-Dawley
Age Or Age Range:14 weeks
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Dosage
SA01091814NNicotine 6mg/kg
SA01091915NNicotine 6mg/kg
SA01092013NNicotine 6mg/kg
SA01092116NNicotine 6mg/kg
SA01092212NNicotine 6mg/kg
SA01092310NNicotine 6mg/kg
SA0109249NNicotine 6mg/kg
SA01092511NNicotine 6mg/kg
SA01091027NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091126NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091225NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091329NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091428NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091532NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091631NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01091730NOCNicotine + Oral contraceptives 6mg/kg Nicotine
SA01092623OCOral contraceptives 1 mL
SA01092724OCOral contraceptives 1 mL
SA01092821OCOral contraceptives 1 mL
SA01092922OCOral contraceptives 1 mL
SA01093020OCOral contraceptives 1 mL
SA01093118OCOral contraceptives 1 mL
SA01093217OCOral contraceptives 1 mL
SA01093319OCOral contraceptives 1 mL
SA0109344SSaline 2 mL
SA0109353SSaline 2 mL
SA0109365SSaline 2 mL
SA0109372SSaline 2 mL
SA0109388SSaline 2 mL
SA0109391SSaline 2 mL
SA0109407SSaline 2 mL
SA0109416SSaline 2 mL
Showing results 1 to 32 of 32

Collection:

Collection ID:CO000244
Collection Summary:At the end of treatments, hippocampus was obtained and immediately frozen in liquid nitrogen
Sample Type:Brain

Treatment:

Treatment ID:TR000264
Treatment Summary:Rats were exposed to the four treatments – saline, nicotine, oral contraceptives and nicotine + oral contraceptives for a month.

Sample Preparation:

Sampleprep ID:SP000258
Sampleprep Summary:-
Sampleprep Protocol Filename:Rat_brain_tissue_prep.pdf
Sampleprep Protocol Comments:Appendix C - Protein and DNA quant.pdf
Organ:Brain

Combined analysis:

Analysis ID AN000353 AN000354
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000262
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Internal Standard:Appendix A - Internal Standard Prep GLCMS.pdf
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

MS:

MS ID:MS000299
Analysis ID:AN000353
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000300
Analysis ID:AN000354
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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