Summary of study ST000237

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Study IDST000237
Study TitleQuick Comparison of Serum Metabolites in SD Rats of Different Sex by Untargeted UPLC-TOFMS and In-house Software Platform
Study TypeBiomarker Discovery
Study SummaryMale (n=8) and female (n=8) SD rats weighing between 220 and 250g were used. On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. Then a blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum. All urine and serum samples were frozen at -80°C prior to analysis.
InstituteBeijing Institute of Radiation Medicine
DepartmentDepartment of Pharmacology and Toxicology
Last NameLiang
First NameQiande
Address27 Taiping Road, Beijing, P.R.China
Emailliangqiande@yeah.net
Submit Date2015-08-09
Num Groups2
Total Subjects16
Release Date2015-09-09
Release Version1

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Project:

Project ID:PR000190
Project DOI:doi: 10.21228/M8Q30F
Project Title:Quick Comparison of Metabolites in Human and Rats of Different Sex by Untargeted UPLC-TOFMS and In-house Software Platform_2
Project Type:Biomarker Discovery
Project Summary:Metabolite difference between sexes has rarely been observed in global manner. In this study, untargeted UPLC-TOFMS and an in-house software platform were used for quick comparison of sex difference of urinary metabolites in human, and of urinary and serum metabolites in SD rats. In addition, as a convenient opportunity, the species difference of urinary metabolites between human and SD rats were also observed. Human urine samples were collected before breakfast from 14 male and 13 female Chinese post-graduate students, age from 23 to 29, on the morning of sample collection day. Male (n=8) and female (n=8) SD rats weighing between 220 and 250g were used. On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. Then a blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum. All urine and serum samples were frozen at -80°C prior to analysis. The study of sex differences is important for finding the best course of treatment of disease as well as for developing novel targets of therapy. A more complete understanding of the metabolic differences across sex-based subgroups is helpful to improve the mechanistic understanding of sex differences.
Institute:Beijing Institute of Radiation Medicine
Department:Department of Pharmacology and Toxicology
Last Name:Liang
First Name:Qiande
Address:27 Taiping Road, Beijing, P.R.China
Email:liangqiande@yeah.net
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