Summary of study ST000239

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000192. The data can be accessed directly via it's Project DOI: 10.21228/M8K88Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000239
Study TitleSexual antagonism in exuded non-volatile metabolites in C. purpureus
Study Typemale - female
Study SummaryThe experimental approach seeks to test for sexual dimorphism in exuded non-volatile metabolites in C. purpureus. The proposed research is creative and original both in its inter-disciplinary approach and its use of a biochemically tractable phenotype to develop a much-needed link between natural selection for sexual dimorphism and the molecular targets of that selection pressure.
Institute
University of Florida
DepartmentSECIM
Last NameMcDaniel
First NameStuart
Emailstuartmcdaniel@ufl.edu
Submit Date2015-07-01
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Stuart McDaniel Stuart McDaniel
https://dx.doi.org/10.21228/M8K88Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000192
Project DOI:doi: 10.21228/M8K88Z
Project Title:Sexual antagonism in exuded non-volatile metabolites in C. purpureus
Project Summary:To study the mechanisms responsible for the evolution of sexual dimorphism by identifying sex differences in metabolite production. The PIs recently demonstrated that female mosses produce large numbers of volatile organic compounds (VOCs) that specifically attract microarthropods, and that the presence of these insects increases fertilization rates (ie, a previously unknown fertilization syndrome). Male mosses produce many fewer VOCs, suggesting that VOC production may be costly. Collectively, these results suggest that alleles controlling VOC production may experience ongoing sexual antagonism. Here the PIs propose to generate pilot data to rigorously establish a link between dimorphisms in metabolite profile and its underlying genetic bases in the moss Ceratodon purpureus and related species.
Institute:University of Florida;Pennsylvania State University
Department:Biology
Last Name:McDaniel
First Name:Stuart
Email:stuartmcdaniel@ufl.edu
Funding Source:Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209

Subject:

Subject ID:SU000259
Subject Type:Plant
Subject Species:Ceratodon purpurues
Taxonomy ID:3225
Species Group:Plant

Factors:

Subject type: Plant; Subject species: Ceratodon purpurues (Factor headings shown in green)

mb_sample_id local_sample_id Gender
SA011092I180fFemale
SA011093H140fFemale
SA011094B150fFemale
SA011095O120fFemale
SA011096N170fFemale
SA011097G150fFemale
SA011098L100fFemale
SA011099E270fFemale
SA011100M280fFemale
SA011101F140fFemale
SA011102M270mMale
SA011103G100mMale
SA011104H150mMale
SA011105I190mMale
SA011106E280mMale
SA011107B190mMale
SA011108F110mMale
SA011109L120mMale
SA011110O110mMale
SA011111N110mMale
Showing results 1 to 20 of 20

Collection:

Collection ID:CO000249
Collection Summary:Flash frozen and lyophilzed
Sample Type:Plant

Treatment:

Treatment ID:TR000269
Treatment Summary:standard growth conditions - 6 day old protonema

Sample Preparation:

Sampleprep ID:SP000263
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol.pdf

Combined analysis:

Analysis ID AN000369 AN000370
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000268
Methods Filename:Metabolomics_LCMSProtocol.pdf
Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000315
Analysis ID:AN000369
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000316
Analysis ID:AN000370
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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