Summary of study ST000248

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000200. The data can be accessed directly via it's Project DOI: 10.21228/M8JC79 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000248
Study TitleMetabolic heterogeneity in Glioblastoma
Study TypeMultiple patient-derived cell lines screening
Study Summary2 cell populations [slow and fast-cycling cells] were isolated from 3 different patient-derived glioblastoma stem cell lines [L0, L1, L2].
Institute
University of Florida
DepartmentSECIM
LaboratoryGarrett Lab
Last NameDeleyrolle
First NameLoic
AddressR3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Emaill.deleyrolle@gmail.com
Submit Date2015-03-24
Num Groups2
Total Subjects6
Study CommentsLine names: L0, L1 & L2. Subpopulation names: slow-cycling cells [S], fast-cycling cell [F]. Sample list: L0-S, L0-F, L1-S, L1-F, L2-S, L2-F
Raw Data AvailableYes
Raw Data File Type(s).mzXML
Uploaded File Size1 GB
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Loic Deleyrolle Loic Deleyrolle
https://dx.doi.org/10.21228/M8JC79
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000200
Project DOI:doi: 10.21228/M8JC79
Project Title:Metabolic heterogeneity in Glioblastoma
Project Summary:Glioblastoma (GB) is the most common and complex primary brain tumor in adults and has a dismal prognosis, which is attributed largely to the extreme heterogeneity in the cells that make up the cancer and the continual molecular, genetic and metabolic adaptations driving tumor initiation, propagation and resistance to conventional treatments. The most important clinical target to prevent these mechanisms of initiation, propagation and disease recurrence may be a subset of tumor cells, cancer stem cells. Hence, identifying targetable key features of this population is of great interest for the elaboration of strategies to prevent disease initiation and propagation as well as recurrence post treatments. In response to i) the limited success to treat GB that remains universally fatal, ii) the evidences pointing to tumor heterogeneity as the greatest obstacle to achieve therapeutic efficacy and iii) the increasing understanding and importance of bioenergetics in tumor biology and the critical need to integrate metabolism into treatment paradigms, we propose a new model residing in the unique and unprecedented hypothesis of an association between GB management, a distinct slow-cycling cancer stem cell subpopulation and metabolic targeting.
Institute:University of Florida
Department:McKnight Brain Institute, Neurosurgery
Last Name:Deleyrolle
First Name:Loic
Address:#N/A
Email:l.deleyrolle@gmail.com
Phone:352-682-1961

Subject:

Subject ID:SU000268
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell population
SA011378L1-fastFast-cycling cells
SA011379L2-fastFast-cycling cells
SA011380L0-fastFast-cycling cells
SA011381L2-slowSlow-cyling cells
SA011382L1-slowSlow-cyling cells
SA011383L0-slowSlow-cyling cells
Showing results 1 to 6 of 6

Collection:

Collection ID:CO000259
Collection Protocol Filename:Deleyrolle_Collection.txt
Sample Type:Cells

Treatment:

Treatment ID:TR000279
Treatment Protocol Filename:Deleyrolle_Treatment_File.txt

Sample Preparation:

Sampleprep ID:SP000273
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol.pdf
Sampleprep Protocol Comments:Appendix A - Internal Standard Prep GLCMS.pdf

Combined analysis:

Analysis ID AN000391 AN000392
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak height peak height

Chromatography:

Chromatography ID:CH000278
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000335
Analysis ID:AN000391
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000336
Analysis ID:AN000392
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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