Summary of study ST000271

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000215. The data can be accessed directly via it's Project DOI: 10.21228/M8M59M This work is supported by NIH grant, U2C- DK119886.

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Study IDST000271
Study TitleC13 Pyruvate Flux in 3t3 L1
Study Type13C mass isotopomer analysis (flux studies)
Study SummaryPyruvate isotopically labeled by 13C either at position 1 or 2 was used to investigate carbon flux in 3T3-L1 mouse cells line in the presence of various combinations of drugs
Institute
University of Michigan
DepartmentMolecular and Integrative Physiology (MCTP)
LaboratoryMacdougald Lab (MCTP)
Last NameMacDougald
First NameOrmond
AddressUniversity Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Emailmacdouga@umich.edu
Phone734-647-4880
Submit Date2014-01-21
Num Groups10
Total Subjects10
Analysis Type DetailLC-MS
Release Date2016-01-06
Release Version1
Ormond MacDougald Ormond MacDougald
https://dx.doi.org/10.21228/M8M59M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000215
Project DOI:doi: 10.21228/M8M59M
Project Title:13C mass isotopomer analysis (flux studies)
Project Summary:13 Flux in lipid metabolism
Institute:University of Michigan
Department:Molecular and Integrative Physiology (MCTP)
Laboratory:Macdougald Lab
Last Name:MacDougald
First Name:Ormond
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:macdouga@umich.edu
Phone:734-647-4880

Subject:

Subject ID:SU000291
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Drug combination 13C-label position in pyruvate
SA012142S00012694Isoproterenol C1
SA012143S00012695Isoproterenol C2
SA012140S00012692Isoproterenol + Leelamine C1
SA012141S00012693Isoproterenol + Leelamine C2
SA012144S00012688None C1
SA012145S00012689None C2
SA012148S00012696Rosiglitazone C1
SA012149S00012697Rosiglitazone C2
SA012146S00012690Rosiglitazone + Leelamine C1
SA012147S00012691Rosiglitazone + Leelamine C2
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000285
Sample Type:Cell Line

Treatment:

Treatment ID:TR000305

Sample Preparation:

Sampleprep ID:SP000299
Sampleprep Protocol Filename:Gly-TCA-nucleotides_analysis_protocol-2015-03-09.docx

Combined analysis:

Analysis ID AN000434
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1260
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520 QTOF
Ion Mode NEGATIVE
Units Area

Chromatography:

Chromatography ID:CH000303
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm-No_Insert.m
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS000375
Analysis ID:AN000434
Instrument Name:Agilent 6520 QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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