Summary of Study ST000272
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000216. The data can be accessed directly via it's Project DOI: 10.21228/M8GC7P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000272 |
| Study Title | Acyl-carnitine analysis (plasma) |
| Study Type | Adipose Acyl-carnitines |
| Study Summary | To understand the contribution of adipose tissue fatty acid oxidation to whole-body metabolism, we generated mice with an adipose-specific knockout of carnitine palmitoyltransferase 2 (CPT2A?/?), an obligate step in mitochondrial long-chain fatty acid oxidation. CPT2A?/? mice became hypothermic after an acute cold challenge, and CPT2A?/? brown adipose tissue (BAT) failed to upregulate thermogenic genes in response to agonist-induced stimulation. The adipose-specific loss of CPT2 resulted in diet-dependent changes in adiposity but did not result in changes in body weight on low- or high-fat diets. Additionally, CPT2A?/? mice had suppressed high-fat diet-induced oxidative stress and inflammation in visceral white adipose tissue (WAT); however, high-fat diet-induced glucose intolerance was not improved. These data show that fatty acid oxidation is required for cold-induced thermogenesis in BAT and high-fat diet-induced oxidative stress and inflammation in WAT. http://www.sciencedirect.com/science/article/pii/S2211124714010523 |
| Institute | University of Michigan |
| Department | Biological Chemistry(Johns Hopkins University School of Medicine) |
| Laboratory | Wolfgang Lab(Johns Hopkins University School of Medicine) |
| Last Name | Wolfgang |
| First Name | Michael |
| Address | 733 North Broadway, Suite G49 Baltimore, MD 21205-2196 |
| mwolfga1@jhmi.edu | |
| Phone | 443-287-7680 |
| Submit Date | 2014-02-21 |
| Num Groups | 2 |
| Total Subjects | 16 |
| Study Comments | (CPT2A?/?) stands for Carnitine palmitoyltransferase 2 knock out |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-01-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000216 |
| Project DOI: | doi: 10.21228/M8GC7P |
| Project Title: | Adipose Oxidation |
| Project Summary: | Role of Fatty Acid Oxidation in Adipose Tissue |
| Institute: | Johns Hopkins University |
| Department: | Biological Chemistry |
| Laboratory: | Wolfgang Lab |
| Last Name: | Wolfgang |
| First Name: | Michael |
| Address: | 733 North Broadway, Suite G49 Baltimore, MD 21205-2196 |
| Email: | mwolfga1@jhmi.edu |
| Phone: | 443-287-7680 |
Subject:
| Subject ID: | SU000292 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild Type and CPT2A-/-. |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA012150 | S00012837 | (CPT2A?/?) |
| SA012151 | S00012836 | (CPT2A?/?) |
| SA012152 | S00012835 | (CPT2A?/?) |
| SA012153 | S00012838 | (CPT2A?/?) |
| SA012154 | S00012839 | (CPT2A?/?) |
| SA012155 | S00012841 | (CPT2A?/?) |
| SA012156 | S00012840 | (CPT2A?/?) |
| SA012157 | S00012834 | (CPT2A?/?) |
| SA012158 | S00012833 | Wild type |
| SA012159 | S00012828 | Wild type |
| SA012160 | S00012827 | Wild type |
| SA012161 | S00012829 | Wild type |
| SA012162 | S00012830 | Wild type |
| SA012163 | S00012832 | Wild type |
| SA012164 | S00012831 | Wild type |
| SA012165 | S00012826 | Wild type |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000286 |
| Collection Summary: | - |
| Sample Type: | Tissues |
Treatment:
| Treatment ID: | TR000306 |
Sample Preparation:
| Sampleprep ID: | SP000300 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | Acyl-carnitines_analysis_protocol-CR-MK-20150528.docx |
Chromatography:
| Chromatography ID: | CH000304 |
| Methods ID: | AQM050 |
| Methods Filename: | QM-004-Xbridg2mm_ACar+_MRM-Insert.m |
| Instrument Name: | Agilent 1200 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN000435 |
| Laboratory Name: | MRC2 (University of Michigan) |
| Analysis Type: | MS |
| Acquisition ID: | AQM050 |
| Acquisition Parameters File: | QM-004-Xbridg2mm_ACar+_MRM-Insert.m |
| Instrument Name: | Agilent QQQ 6490 |
| Processing Parameters File: | EX00250-acyl-carnitines-quant.m |
| Chromatography ID: | CH000304 |
| Num Factors: | 2 |
| Num Metabolites: | 30 |
| Units: | pmol/mg sample |
