Summary of study ST000300

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000241. The data can be accessed directly via it's Project DOI: 10.21228/M8H88B This work is supported by NIH grant, U2C- DK119886.

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Study IDST000300
Study TitleChicken/C. jejuni Bile Acid Study
Study SummaryCecal samples were isolated directly from the ceca of dissected chickens that were either experimentally infected with C. jejuni DRH212 or mock-infected with PBS. Cecal samples were re-suspended in Life Technologies 1X PBS based on weight of sample (1 ml/100 mg = 10-1 dilution) and stored at -80C.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-17
Num Groups2
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s).xml, .bin, .xsd
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8H88B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000241
Project DOI:doi: 10.21228/M8H88B
Project Title:Identifiying Colonization Factors of Campylobacter jejuni
Project Summary:Using next-generation approaches to identify both bacterial and host factors that influence colonization of the chicken gastrointestinal tract by C. jejuni
Institute:University of Michigan
Department:Microbiology and Immunology
Laboratory:DiRita Lab
Last Name:DiRita
First Name:Victor
Address:Ann Arbor, MI
Email:vdirita@umich.edu
Phone:734-936-3804

Subject:

Subject ID:SU000320
Subject Type:Animal
Subject Species:Gallus gallus
Taxonomy ID:9031
Species Group:Bird

Factors:

Subject type: Animal; Subject species: Gallus gallus (Factor headings shown in green)

mb_sample_id local_sample_id C. jejuni Infection
SA013604S00017777no
SA013605S00017776no
SA013606S00017775yes
SA013607S00017774yes
Showing results 1 to 4 of 4

Collection:

Collection ID:CO000314
Collection Summary:-
Sample Type:Cecum

Treatment:

Treatment ID:TR000334
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000328
Sampleprep Summary:-
Sampleprep Protocol Filename:Bile Acids-20150305.docx

Combined analysis:

Analysis ID AN000478
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters Acquity BEH C18 (50 x 2mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410 QQQ
Ion Mode NEGATIVE
Units uM

Chromatography:

Chromatography ID:CH000341
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-I.m.zip
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C18 (50 x 2mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000418
Analysis ID:AN000478
Instrument Name:Agilent 6410 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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