Summary of study ST000303

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000137. The data can be accessed directly via it's Project DOI: 10.21228/M8859H This work is supported by NIH grant, U2C- DK119886.

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Study IDST000303
Study TitleHuman fecal bile acid profiles before and after fecal transplant (Part 2)
Study SummaryUnderstand the bile acid profiles from the feces of fecal microbiota transplant FMT patients that successfully recover from recurrent C. difficile infection. Submitting fecal samples from patients prior to their FMT and post FMT. Interested in the bile acid profiles of the donor stool that is used in successful transplants. Bile acids are important for C. difficile spore germination and outgrowth.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-04-28
Num Groups6
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s).xml, .bin, .xsd
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8859H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000137
Project DOI:doi: 10.21228/M8859H
Project Title:Fecal Transplant Studies
Project Summary:Alterations in fecal bile acids before and after fecal transplant
Institute:University of Michigan
Department:Int Med-Infectious Diseases
Laboratory:Theriot Lab
Last Name:Theriot
First Name:Casey
Address:Ann Arbor, MI
Email:caseythe@umich.edu
Phone:734-763-4133

Subject:

Subject ID:SU000323
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Transplant type
SA013690S00018185donor homogenized
SA013691S00018184donor non-homogenized
SA013692S00018194mouse cecum
SA013693S00018193mouse cecum
SA013694S00018191mouse ileum
SA013695S00018192mouse ileum
SA013696S00018190post-FMT
SA013697S00018189post-FMT
SA013698S00018188post-FMT
SA013699S00018181post-FMT
SA013700S00018182post-FMT
SA013701S00018183post-FMT
SA013702S00018187pre-FMT
SA013703S00018186pre-FMT
SA013704S00018180pre-FMT
Showing results 1 to 15 of 15

Collection:

Collection ID:CO000317
Collection Summary:-
Sample Type:Cecum

Treatment:

Treatment ID:TR000337
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000331
Sampleprep Summary:-
Sampleprep Protocol Filename:Bile_Acids-20150305.docx

Combined analysis:

Analysis ID AN000482
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters Acquity BEH C18 (50 x 2mm, 1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode NEGATIVE
Units ug/100 mg

Chromatography:

Chromatography ID:CH000345
Methods Filename:2014-07-30-Bile_acid-full-BEH-C18-I.m.zip
Instrument Name:Agilent 1260
Column Name:Waters Acquity BEH C18 (50 x 2mm, 1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000422
Analysis ID:AN000482
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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