Summary of Study ST000310

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000249. The data can be accessed directly via it's Project DOI: 10.21228/M8M01V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Show all samples  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST000310
Study TitleTC and B6 untreated plasma in lupus-prone mice lipidomics (part-II)
Study Typeuntreated
Study Summarycompare plasma samples from 3 month old lupus-prone (TC) and control (B6) mice
Institute
University of Florida
DepartmentSECIM
LaboratoryLaurence M. Morel Laboratory
Last NameMorel
First NameLaurence
AddressCollege of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Emailmorel@ufl.edu
Phone352-392-3790
Submit Date2016-01-17
Num Groups2
Total Subjects17
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2016-01-18
Release Version1
Laurence Morel Laurence Morel
https://dx.doi.org/10.21228/M8M01V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000249
Project DOI:doi: 10.21228/M8M01V
Project Title:Lupus lipidomics
Project Summary:The purpose of this study was to compare the metabolic and lipidomic profile of plasma from 3 month old lupus-prone (TC) and control (B6) mice.
Institute:University of Florida
Department:Pathology, Immunology and Laboratory Medicine
Laboratory:Laurence M. Morel Laboratory
Last Name:Morel
First Name:Laurence
Address:College of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Email:morel@ufl.edu
Phone:352-392-3790

Subject:

Subject ID:SU000330
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain Treatment
SA014047TC_f3649_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014048TC_f3649B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014049TC_f3646_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014050TC_f3651B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014051TC_f3651_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014052TC_f3668_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014053TC_f3668B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014054TC_f3646B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014055B6_F5_2C57BL/6J control
SA014056B6_F3C57BL/6J control
SA014057B6_F2C57BL/6J control
SA014058B6_F1_2C57BL/6J control
SA014059B6_F3_2C57BL/6J control
SA014060B6_F4C57BL/6J control
SA014061B6_F5C57BL/6J control
SA014062B6_F4_2C57BL/6J control
SA014063B6_F1C57BL/6J control
Showing results 1 to 17 of 17

Collection:

Collection ID:CO000324
Collection Summary:None
Collection Protocol ID:IACUC 201308189
Sample Type:blood
Collection Method:heart puncture
Collection Time:terminal
Storage Conditions:-20C
Collection Vials:heparinized tubes
Blood Serum Or Plasma:plasma

Treatment:

Treatment ID:TR000344
Treatment Summary:no treatement (controls for KD diet study)

Sample Preparation:

Sampleprep ID:SP000338
Sampleprep Summary:None
Sampleprep Protocol Filename:Lipidomics_LCMSProtocol.pdf

Chromatography:

Chromatography ID:CH000350
Chromatography Summary:None
Methods Filename:Lipidomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN000490
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000350
Num Factors:2
Num Metabolites:921
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak Area
  
Analysis ID:AN000491
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000350
Num Factors:2
Num Metabolites:90
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak Area
  
Analysis ID:AN000492
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000350
Num Factors:2
Num Metabolites:921
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak height
  
Analysis ID:AN000493
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000350
Num Factors:2
Num Metabolites:90
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak height
  logo