Summary of Study ST000311

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000250. The data can be accessed directly via it's Project DOI: 10.21228/M8G881 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000311
Study TitleTC and B6 untreated plasma in lupus-prone mice
Study Typeuntreated
Study Summarycompare plasma samples from 3 month old lupus-prone (TC) and control (B6) mice
Institute
University of Florida
DepartmentSECIM
LaboratoryLaurence M. Morel Laboratory
Last NameMorel
First NameLaurence
AddressCollege of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Emailmorel@ufl.edu
Phone352-392-3790
Submit Date2016-01-17
Num Groups2
Total Subjects17
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-02-01
Release Version1
Laurence Morel Laurence Morel
https://dx.doi.org/10.21228/M8G881
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000250
Project DOI:doi: 10.21228/M8G881
Project Title:Lupus metabolomics
Project Summary:The purpose of this study was to compare the metabolic and lipidomic profile of plasma from 3 month old lupus-prone (TC) and control (B6) mice.
Institute:University of Florida
Department:Pathology, Immunology and Laboratory Medicine
Laboratory:Laurence M. Morel Laboratory
Last Name:Morel
First Name:Laurence
Address:College of Medicine 1395 Center Drive, D6-18 Gainesville, FL 32610-0275
Email:morel@ufl.edu
Phone:352-392-3790

Subject:

Subject ID:SU000331
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:[C57BL-6J (B6)]/[B6.Sle1.Sle2.Sle3 (TC)]
Age Or Age Range:99 days/93-99 days
Gender:female
Animal Animal Supplier:in house/in house
Animal Housing:SPF/SPF
Animal Light Cycle:standard|standard
Animal Feed:standard|standard
Animal Water:standard|standard
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Strain Treatment
SA014064TC_f3649_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014065TC_f3649B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014066TC_f3646_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014067TC_f3651B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014068TC_f3651_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014069TC_f3668_2B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014070TC_f3668B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014071TC_f3646B6.Sle1.Sle2.Sle3 (TC) lupus prone
SA014072B6_F5_2C57BL/6J control
SA014073B6_F3C57BL/6J control
SA014074B6_F2C57BL/6J control
SA014075B6_F1_2C57BL/6J control
SA014076B6_F3_2C57BL/6J control
SA014077B6_F4C57BL/6J control
SA014078B6_F5C57BL/6J control
SA014079B6_F4_2C57BL/6J control
SA014080B6_F1C57BL/6J control
Showing results 1 to 17 of 17

Collection:

Collection ID:CO000325
Collection Summary:None
Collection Protocol ID:IACUC 201308189
Collection Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf
Sample Type:blood
Collection Method:heart puncture
Collection Time:terminal
Storage Conditions:-20C
Collection Vials:heparinized tubes
Blood Serum Or Plasma:plasma

Treatment:

Treatment ID:TR000345
Treatment Summary:no treatement (controls for KD diet study)

Sample Preparation:

Sampleprep ID:SP000339
Sampleprep Summary:None
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf
Appendix A - Internal Standard Prep GLCMS.pdf

Chromatography:

Chromatography ID:CH000351
Chromatography Summary:None
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Flow Rate:0.350mL/min-0.600mL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN000494
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000351
Num Factors:2
Num Metabolites:680
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak Area
  
Analysis ID:AN000495
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000351
Num Factors:2
Num Metabolites:723
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak Area
  
Analysis ID:AN000496
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000351
Num Factors:2
Num Metabolites:680
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak height
  
Analysis ID:AN000497
Analysis Type:MS
Analysis Protocol File:Metabolomics_LCMSProtocol_plasma.pdf
Instrument Name:Thermo Scientific Q-Exactive
Chromatography ID:CH000351
Num Factors:2
Num Metabolites:723
Has Mz:1
Has Rt:1
Rt Units:Minutes
Units:Peak height
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