Summary of Study ST000312

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000251. The data can be accessed directly via it's Project DOI: 10.21228/M8BG63 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000312
Study TitleIDH1R132H activity in glioma cell lines and tumnor tissue (2HG)
Study TypeRegular
Study SummaryWe developed genetically engineered mice to generate brain tumors with especific genetic lessions. The animals were split in three groups: NRAS, P53 knockdown, IDH1-R132H and ATRX knock down (NPAID); NRAS, P53 knockdown, IDH1-R132H (NPI); NRAS, P53 knockdown, (NshP53). From these tumor we obtain and culture tumor cells growth like neurospheres and attached cells.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-05
Num Groups4
Total Subjects4
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8BG63
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000251
Project DOI:doi: 10.21228/M8BG63
Project Title:IDH1 gliomas
Project Summary:Analyze IDH1R132H activity in glioma cell lines and tumnor tissue
Institute:University of Michigan
Department:Neurosurgery
Laboratory:Castro Lab
Last Name:Castro
First Name:Maria
Address:Ann Arbor, MI
Email:mariacas@umich.edu
Phone:734-764-0850

Subject:

Subject ID:SU000332
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genetic makeup
SA014081S00017646NPAID
SA014082S00017647NPI
SA014083S00017648NshP53
SA014084S00020347Pooled cells
Showing results 1 to 4 of 4

Collection:

Collection ID:CO000326
Collection Summary:None provided.
Sample Type:Cells

Treatment:

Treatment ID:TR000346
Treatment Summary:None provided.

Sample Preparation:

Sampleprep ID:SP000340
Sampleprep Summary:None provided.
Sampleprep Protocol Filename:15DEC2015_2HG_cells_Castro.docx

Combined analysis:

Analysis ID AN000498
Analysis type MS
Chromatography type Reversed phase
Chromatography system Unspecified
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode NEGATIVE
Units µM/500µl extraction solvent

Chromatography:

Chromatography ID:CH000352
Methods ID:DQM0033
Methods Filename:2015-04-07-2HG.m.zip
Instrument Name:Unspecified
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000436
Analysis ID:AN000498
Instrument Name:Agilent 6410A QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:NEGATIVE
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