Summary of Study ST000325
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000262. The data can be accessed directly via it's Project DOI: 10.21228/M8X882 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000325 |
| Study Title | Metabolomic effects of metformin on mouse liver, intestine, and serum |
| Study Summary | Experiment to test the different metabolomic effects of two different doses of metformin (50mg vs 150 mg). A saline treatment group was used as a control. The effects were measured at the liver, intestine, and serum of the mouse. |
| Institute | University of California, Davis |
| Department | Genome and Biomedical Sciences Facility |
| Laboratory | WCMC Metabolomics Core |
| Last Name | Fiehn |
| First Name | Oliver |
| Address | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
| ofiehn@ucdavis.edu | |
| Phone | (530) 754-8258 |
| Submit Date | 2015-03-16 |
| Num Groups | 7 |
| Total Subjects | 53 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000262 |
| Project DOI: | doi: 10.21228/M8X882 |
| Project Title: | Metabolomic effects of metformin on mouse liver, intestine, and serum |
| Project Summary: | Experiment to test the different metabolomic effects of two different doses of metformin (50mg vs 150mg). A saline treatment group was used as a control. The effects were measured at the liver, intestine, and serum of the mouse. |
| Institute: | University of California, Davis |
| Department: | Genome and Biomedical Sciences Facility |
| Laboratory: | WCMC Metabolomics Core |
| Last Name: | Fiehn |
| First Name: | Oliver |
| Address: | 1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616 |
| Email: | ofiehn@ucdavis.edu |
| Phone: | (530) 754-8258 |
| Funding Source: | U24DK097154 |
Subject:
| Subject ID: | SU000345 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Organ | Treatment |
|---|---|---|---|
| SA014662 | Metformin150mg/kg-1I-2meta_intestine | intestine | Metformin 150 mg |
| SA014663 | Metformin150mg/kg-5I-2meta_intestine | intestine | Metformin 150 mg |
| SA014664 | Metformin150mg/kg-4I-2meta_intestine | intestine | Metformin 150 mg |
| SA014665 | Metformin150mg/kg-2I-2meta_intestine | intestine | Metformin 150 mg |
| SA014666 | Metformin150mg/kg-3I-2meta_intestine | intestine | Metformin 150 mg |
| SA014667 | Metformin150mg/kg-6I-2meta_intestine | intestine | Metformin 150 mg |
| SA014668 | Metformin50mg/kg-2I-2meta_intestine | intestine | Metformin 50 mg |
| SA014669 | Metformin50mg/kg-6I-2meta_intestine | intestine | Metformin 50 mg |
| SA014670 | Metformin50mg/kg-5I-2meta_intestine | intestine | Metformin 50 mg |
| SA014671 | Metformin50mg/kg-4I-2meta_intestine | intestine | Metformin 50 mg |
| SA014672 | Metformin50mg/kg-3I-2meta_intestine | intestine | Metformin 50 mg |
| SA014673 | Metformin50mg/kg-1I-2meta_intestine | intestine | Metformin 50 mg |
| SA014674 | Saline-4I-2meta_intestine | intestine | Saline |
| SA014675 | Saline-6I-2meta_intestine | intestine | Saline |
| SA014676 | Saline-3I-2meta_intestine | intestine | Saline |
| SA014677 | Saline-2I-2meta_intestine | intestine | Saline |
| SA014678 | Saline-5I-2meta_intestine | intestine | Saline |
| SA014679 | Saline-1I-2meta_intestine | intestine | Saline |
| SA014680 | Metformin150mg/kg-2_liver | liver | Metformin 150 mg |
| SA014681 | Metformin150mg/kg-1_liver | liver | Metformin 150 mg |
| SA014682 | Metformin150mg/kg-3_liver | liver | Metformin 150 mg |
| SA014683 | Metformin150mg/kg-5_liver | liver | Metformin 150 mg |
| SA014684 | Metformin150mg/kg-4_liver | liver | Metformin 150 mg |
| SA014685 | Metformin150mg/kg-6_liver | liver | Metformin 150 mg |
| SA014686 | Metformin50mg/kg-4_liver | liver | Metformin 50 mg |
| SA014687 | Metformin50mg/kg-5_liver | liver | Metformin 50 mg |
| SA014688 | Metformin50mg/kg-2_liver | liver | Metformin 50 mg |
| SA014689 | Metformin50mg/kg-1_liver | liver | Metformin 50 mg |
| SA014690 | Metformin50mg/kg-6_liver | liver | Metformin 50 mg |
| SA014691 | Metformin50mg/kg-3_liver | liver | Metformin 50 mg |
| SA014692 | Saline-4_liver | liver | Saline |
| SA014693 | Saline-6_liver | liver | Saline |
| SA014694 | Saline-5_liver | liver | Saline |
| SA014695 | Saline-3_liver | liver | Saline |
| SA014696 | Saline-2_liver | liver | Saline |
| SA014697 | Saline-1_liver | liver | Saline |
| SA014698 | Metformin150mg/kg-4_serum | serum | Metformin 150 mg |
| SA014699 | Metformin150mg/kg-5_serum | serum | Metformin 150 mg |
| SA014700 | Metformin150mg/kg-2_serum | serum | Metformin 150 mg |
| SA014701 | Metformin150mg/kg-1_serum | serum | Metformin 150 mg |
| SA014702 | Metformin150mg/kg-6_serum | serum | Metformin 150 mg |
| SA014703 | Metformin150mg/kg-3_serum | serum | Metformin 150 mg |
| SA014704 | Metformin50mg/kg-4_serum | serum | Metformin 50 mg |
| SA014705 | Metformin50mg/kg-1_serum | serum | Metformin 50 mg |
| SA014706 | Metformin50mg/kg-6_serum | serum | Metformin 50 mg |
| SA014707 | Metformin50mg/kg-5_serum | serum | Metformin 50 mg |
| SA014708 | Metformin50mg/kg-3_serum | serum | Metformin 50 mg |
| SA014709 | Metformin50mg/kg-2_serum | serum | Metformin 50 mg |
| SA014710 | Saline-2_serum | serum | Saline |
| SA014711 | Saline-1_serum | serum | Saline |
| SA014712 | Saline-3_serum | serum | Saline |
| SA014713 | Saline-5_serum | serum | Saline |
| SA014714 | Saline-6_serum | serum | Saline |
| SA014715 | Saline-4_serum | serum | Saline |
| Showing results 1 to 54 of 54 |
Collection:
| Collection ID: | CO000339 |
| Collection Summary: | - |
| Sample Type: | Serum, liver, intestine |
Treatment:
| Treatment ID: | TR000359 |
| Treatment Summary: | There are 3 different treatment groups (saline and two different doses of metformin). Each group has 6 mice. |
| Treatment Protocol Filename: | StudyDesign_SookWahYee_7914.pdf |
Sample Preparation:
| Sampleprep ID: | SP000353 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | SOP_Extraction_of_Blood_Samples.pdf SOP_Extraction_of_Liver_Tissue_Samples.pdf |
Chromatography:
| Chromatography ID: | CH000363 |
| Chromatography Summary: | UPLC |
| Methods Filename: | Data_Dictionary_Fiehn_laboratory_HILIC_QTOF_MS.pdf |
| Instrument Name: | Unspecified |
| Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Column Pressure: | 200-700 bar |
| Column Temperature: | 45 C |
| Flow Gradient: | 100% B to 30% B |
| Flow Rate: | 0.4 mL/min |
| Internal Standard: | See data dictionary |
| Retention Time: | See data dictionary |
| Sample Injection: | 3 uL |
| Analytical Time: | 14 min |
| Capillary Voltage: | 4500 |
| Time Program: | 16.75 min |
| Weak Wash Solvent Name: | 1:1 ACN:H2O |
| Weak Wash Volume: | 5 seconds |
| Strong Wash Solvent Name: | 1:1 ACN:H2O |
| Target Sample Temperature: | Autosampler temp 4 C |
| Randomization Order: | Excel |
| Chromatography Type: | HILIC |
Analysis:
| Analysis ID: | AN000518 |
| Laboratory Name: | WCMC Metabolomics Core |
| Analysis Type: | MS |
| Instrument Name: | Agilent 6530 QTOF |
| Software Version: | MassHunter |
| Detector Type: | TOF MCP |
| Data Format: | .d |
| Chromatography ID: | CH000363 |
| Num Factors: | 9 |
| Num Metabolites: | 28 |
| Rt Units: | Minutes |
| Units: | Peak height |
