Summary of Study ST000344

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000276. The data can be accessed directly via it's Project DOI: 10.21228/M83W3X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000344
Study TitleEffects of dietary supplement on hamster metabolism
Study SummaryThis experiment aims to analyze spent media from a protein over-expression system. The treatment was a lipid supplement given to hamsters. The spent media was then analyzed to see how the lipid supplement affected lipid metabolism.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2016-02-09
Num Groups6
Total Subjects28
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-03-03
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M83W3X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000276
Project DOI:doi: 10.21228/M83W3X
Project Title:Effects of dietary supplement on hamster metabolism
Project Summary:This experiment aims to analyze spent media from a protein over-expression system. The treatment was a lipid supplement given to hamsters. The spent media was then analyzed to see how the lipid supplement affected lipid metabolism.
Institute:University of California, Davis
Department:Genome and Biomedical Sciences Facility
Laboratory:WCMC Metabolomics Core
Last Name:Fiehn
First Name:Oliver
Address:1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Email:ofiehn@ucdavis.edu
Phone:(530) 754-8258
Funding Source:NIH U24DK097154

Subject:

Subject ID:SU000364
Subject Type:Animal
Subject Species:Mesocricetus auratus
Taxonomy ID:10036
Species Group:Mammals

Factors:

Subject type: Animal; Subject species: Mesocricetus auratus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA015262CHO cells_Elhofy_d14 C_005.dControl
SA015263CHO cells_Elhofy_d17 C_006.dControl
SA015264CHO cells_Elhofy_d2 C_001.dControl
SA015265CHO cells_Elhofy_d11 C_004.dControl
SA015266CHO cells_Elhofy_d5 C_002.dControl
SA015267CHO cells_Elhofy_d8 C_003.dControl
SA015268CHO cells_Elhofy_d17 E_012.dLipid Supplement
SA015269CHO cells_Elhofy_d2 E_007.dLipid Supplement
SA015270CHO cells_Elhofy_d5 E_008.dLipid Supplement
SA015271CHO cells_Elhofy_d11 E_010.dLipid Supplement
SA015272CHO cells_Elhofy_d8 E_009.dLipid Supplement
SA015273CHO cells_Elhofy_d14 E_011.dLipid Supplement
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000358
Collection Summary:This is spent media from a protein over-expression system. The media is serum free from the DG44 CHO cell line
Collection Protocol Filename:StudyDesignAdamElhofy10202015.pdf
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000378
Treatment Summary:2x6 each: The treatment is the addition of a lipid supplement.
Treatment Protocol Filename:StudyDesignAdamElhofy10202015.pdf

Sample Preparation:

Sampleprep ID:SP000371
Sampleprep Summary:None

Chromatography:

Chromatography ID:CH000394
Chromatography Summary:UPLC
Methods Filename:Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf
Instrument Name:Agilent 6530
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Pressure:450-850 bar
Column Temperature:65 C
Flow Gradient:15% B to 99% B
Flow Rate:0.6 mL/min
Sample Injection:1.67 uL
Solvent A:60% acetonitrile/40% water; 10mM formic acid; 10mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 10mM formic acid; 10mM ammonium formate
Analytical Time:13 min
Capillary Voltage:3500
Time Program:15 min
Weak Wash Solvent Name:Isopropanol
Weak Wash Volume:5 seconds
Strong Wash Solvent Name:Same
Target Sample Temperature:Autosampler temp 4 C
Randomization Order:Excel
Chromatography Type:Reversed phase
  
Chromatography ID:CH000395
Chromatography Summary:UPLC
Methods Filename:Data_Dictionary_Fiehn_laboratory_CSH_QTOF_lipidomics_05-29-2014.pdf
Instrument Name:Agilent 6550
Column Name:Waters Acquity CSH C18 (100 x 2.1mm,1.7um)
Column Pressure:450-850 bar
Column Temperature:65 C
Flow Gradient:15% B to 99% B
Flow Rate:0.6 mL/min
Sample Injection:5.0 uL
Solvent A:40% water/60% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Solvent B:90% isopropanol/10% acetonitrile; 10mM acetic acid; 10mM ammonium acetate
Analytical Time:13 min
Capillary Voltage:3500
Time Program:15 min
Weak Wash Solvent Name:Isopropanol
Weak Wash Volume:5 seconds
Strong Wash Solvent Name:Same
Target Sample Temperature:Autosampler temp 4 C
Randomization Order:Excel
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN000557
Analysis Type:MS
Instrument Name:Agilent/6530 Accurate Mass QTOF (Positive Mode)
Software Version:MassHunter
Detector Type:TOF MCP
Data Format:.d
Chromatography ID:CH000394
Num Factors:2
Num Metabolites:58
Rt Units:Minutes
Units:Peak height
  
Analysis ID:AN000558
Analysis Type:MS
Instrument Name:Agilent/6550 Accurate Mass QTOF (Negative Mode)
Software Version:MassHunter
Detector Type:TOF MCP
Data Format:.d
Chromatography ID:CH000395
Num Factors:2
Num Metabolites:62
Rt Units:Minutes
Units:Peak height
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