Summary of Study ST000409

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000320. The data can be accessed directly via it's Project DOI: 10.21228/M8MP4W This work is supported by NIH grant, U2C- DK119886.

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Study IDST000409
Study TitleMetabolomic analysis of oxytocin effects on social deficits in mice (part II)
Study SummaryThe goal of this study is to determine the prosocial hormone oxytocin (OT) effects on metabolomic profiles in feces.
Institute
University of North Carolina
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-02
Raw Data AvailableYes
Raw Data File Type(s)1r
Analysis Type DetailNMR
Release Date2018-06-05
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8MP4W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000320
Project DOI:doi: 10.21228/M8MP4W
Project Title:Metabolomic analysis of oxytocin effects on social deficits in mice
Project Summary:The goal of this study was to determine the effects of the neuropeptide oxytocin (OT) on metabolomic profiles, using a mouse model of autism-like behavior, the BALB/cByJ inbred strain. We have previously reported that subchronic treatment with OT can lead to persistent reversal of social deficits in BALB/cByJ and other models of autism spectrum disorder (ASD). In this study, mice were given a subchronic regimen with either vehicle or OT, and then evaluated for social approach. At the end of the study, brain and blood were collected for metabolomic analysis. In addition, fecal samples were taken at different time points during the treatment and testing regimen. The results from this project could elucidate mechanisms underlying the prosocial effects of oxytocin, and identify new targets for the development of highly specific oxytocin-related drugs.
Institute:University of North Carolina at Chapel Hill
Department:Department of Psychiatry
Last Name:Moy
First Name:Sheryl
Address:CB# 7146, Chapel Hill, NC 277599
Email:ssmoy@med.unc.edu
Phone:919-966-3082

Subject:

Subject ID:SU000430
Subject Type:Mice
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mice; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Time Point Pool
SA019987F_15OT S1 N
SA019988F_6OT S1 N
SA019989F_3OT S1 N
SA019990F_1OT S1 N
SA019991F_8OT S1 N
SA019992F_10OT S1 N
SA019993F_12OT S1 N
SA019994F_5OT S1 Y
SA019995F_19OT S1 Y
SA019996F_2OT S1 Y
SA019997F_17OT S1 Y
SA019998F_14OT S1 Y
SA019999F_26OT S2 N
SA020000F_32OT S2 N
SA020001F_25OT S2 N
SA020002F_28OT S2 N
SA020003F_37OT S2 N
SA020004F_39OT S2 N
SA020005F_22OT S2 N
SA020006F_35OT S2 Y
SA020007F_34OT S2 Y
SA020008F_30OT S2 Y
SA020009F_23OT S2 Y
SA020010F_21OT S2 Y
SA020011F_41OT S3 N
SA020012F_55OT S3 N
SA020013F_50OT S3 N
SA020014F_45OT S3 N
SA020015F_43OT S3 N
SA020016F_46OT S3 N
SA020017F_48OT S3 N
SA020018F_42OT S3 Y
SA020019F_52OT S3 Y
SA020020F_59OT S3 Y
SA020021F_57OT S3 Y
SA020022F_54OT S3 Y
SA020023F_61OT S4 N
SA020024F_68OT S4 N
SA020025F_77OT S4 N
SA020026F_72OT S4 N
SA020027F_70OT S4 N
SA020028F_65OT S4 N
SA020029F_62OT S4 N
SA020030F_74OT S4 Y
SA020031F_79OT S4 Y
SA020032F_63OT S4 Y
SA020033F_75OT S4 Y
SA020034F_66OT S4 Y
SA020035PF_1Pool Pool Pool
SA020036PF_2Pool Pool Pool
SA020037PF_3Pool Pool Pool
SA020038PF_4Pool Pool Pool
SA020039PF_5Pool Pool Pool
SA020040F_4Veh S1 N
SA020041F_7Veh S1 N
SA020042F_9Veh S1 N
SA020043F_11Veh S1 N
SA020044F_20Veh S1 N
SA020045F_16Veh S1 N
SA020046F_18Veh S1 N
SA020047F_13Veh S1 N
SA020048F_24Veh S2 N
SA020049F_40Veh S2 N
SA020050F_33Veh S2 N
SA020051F_38Veh S2 N
SA020052F_31Veh S2 N
SA020053F_29Veh S2 N
SA020054F_27Veh S2 N
SA020055F_36Veh S2 Y
SA020056F_60Veh S3 N
SA020057F_47Veh S3 N
SA020058F_56Veh S3 N
SA020059F_51Veh S3 N
SA020060F_58Veh S3 N
SA020061F_44Veh S3 N
SA020062F_53Veh S3 Y
SA020063F_49Veh S3 Y
SA020064F_76Veh S4 N
SA020065F_64Veh S4 N
SA020066F_69Veh S4 N
SA020067F_80Veh S4 N
SA020068F_78Veh S4 Y
SA020069F_67Veh S4 Y
SA020070F_71Veh S4 Y
SA020071F_73Veh S4 Y
Showing results 1 to 85 of 85

Collection:

Collection ID:CO000424
Collection Summary:24 hr after the social test, mice were deeply anesthetized with 5% isoflurane, followed by rapid decapitation. Brains were removed, rinsed with ice-cold water, and then rapidly dissected into the following 5 regions: forebrain, midbrain, cerebellum, hindbrain, and olfactory bulbs. The dissected parts were flash-frozen on dry ice and stored at -80o C. Samples were coded by treatment: vehicle-treated mice (n=8) and OT-treated mice (n=12).
Sample Type:Feces

Treatment:

Treatment ID:TR000444
Treatment Summary:BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection.

Sample Preparation:

Sampleprep ID:SP000437
Sampleprep Summary:Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Analysis:

Analysis ID:AN000649
Analysis Type:NMR
Num Factors:16

NMR:

NMR ID:NM000071
Analysis ID:AN000649
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:700 MHz
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