Summary of study ST000415

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000324. The data can be accessed directly via it's Project DOI: 10.21228/M83K6Z This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000415
Study TitleToxicokinetics and Metabolomic Disrupting of the Flame Retardant Mixture Firemaster 550
Study TypeLow Dose and High Dose Exposure to Firemaster (FM) 550
Study SummaryPregnant lab rats (dams) were assigned to three groups: a control group, which was not exposed to Firemaster (FM) 550; a low-dose group, which ingested 100 µg of FM550 once daily throughout pregnancy; and a high-dose group, which ingested 1000 µg FM550 on the same schedule. The placentas were harvested immediately after birth, frozen on dry ice and pulverized in liquid nitrogen via mortar and pestle. The overall objective is to investigate the differences in the metabolic profiles of the placentas in each phenotypic group.
Institute
University of North Carolina
DepartmentRCMRC
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-16
Num Groups3
Total Subjects28
Num Males14
Num Females14
Raw Data AvailableYes
Raw Data File Type(s).cdf
Analysis Type DetailGC-MS
Release Date2018-07-17
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M83K6Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000324
Project DOI:doi: 10.21228/M83K6Z
Project Title:Toxicokinetics and Metabolomic Disrupting of the Flame Retardant Mixture Firemaster 550
Project Type:Metabolome Evaluation
Project Summary:Pregnant lab rats (dams) were assigned to three groups: a control group, which was not exposed to Firemaster (FM) 550; a low-dose group, which ingested 100 µg of FM550 once daily throughout pregnancy; and a high-dose group, which ingested 1000 µg FM550 on the same schedule. The placentas were harvested immediately after birth, frozen on dry ice and pulverized in liquid nitrogen via mortar and pestle. The overall objective is to investigate the differences in the metabolic profiles of the placentas in each phenotypic group.
Institute:North Carolina State University
Department:Center for Human Health and the Environment
Laboratory:WM Keck Center for Behvioral Biology
Last Name:Patisaul
First Name:Heather
Address:Raleigh, NC 27695
Email:hbpatisa@ncsu.edu
Phone:919.515.2011

Subject:

Subject ID:SU000436
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Group Gender of Pup in Placenta
SA02081281980-P3CONTROL F
SA02081381002-P5CONTROL F
SA02081481980-12ACONTROL F
SA02081581987-11ACONTROL F
SA02081681979-10ACONTROL F
SA02081781000-9ACONTROL F
SA02081881979-10BCONTROL M
SA02081981000-9BCONTROL M
SA02082081987-11BCONTROL M
SA02082181002-P6CONTROL M
SA02082281980-P4CONTROL M
SA02082381980-12BCONTROL M
SA02082481014-7AHIGHDOSE F
SA02082581991-6AHIGHDOSE F
SA02082681978-8AHIGHDOSE F
SA02082781983-5AHIGHDOSE F
SA02082881014-7BHIGHDOSE M
SA02082981991-6BHIGHDOSE M
SA02083081983-5BHIGHDOSE M
SA02083181978-8BHIGHDOSE M
SA02083281009-4ALOWDOSE F
SA02083381984-2ALOWDOSE F
SA02083481975-1BLOWDOSE F
SA02083581977-3ALOWDOSE F
SA02083681975-1ALOWDOSE M
SA02083781009-4BLOWDOSE M
SA02083881977-3BLOWDOSE M
SA02083981984-2BLOWDOSE M
Showing results 1 to 28 of 28

Collection:

Collection ID:CO000430
Collection Summary:The placentas were harvested immediately after birth by surgery, then frozen on dry ice and pulverized in liquid nitrogen via mortar and pestle.
Sample Type:Placenta

Treatment:

Treatment ID:TR000450
Treatment Summary:Control group was not exposed; Low-dose group ingested 100 µg of FM550 once daily throughout pregnancy; and High-dose group ingested 1000 µg FM550 on the same schedule.

Sample Preparation:

Sampleprep ID:SP000443
Sampleprep Summary:Aliquots of approximately 50 mg of placental tissue were mixed with 1 mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Combined analysis:

Analysis ID AN000657
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus 4D GC
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH000474
Instrument Name:Leco Pegasus 4D GC
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Column Pressure:12.8psi
Flow Gradient:constant flow
Flow Rate:1ml/min
Injection Temperature:250C
Retention Index:FAME markers C8-C30
Chromatography Type:GC

MS:

MS ID:MS000583
Analysis ID:AN000657
Instrument Name:Leco Pegasus 4D GCxGC TOF
Instrument Type:GC x GC-TOF
MS Type:EI
Ion Mode:POSITIVE
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