Summary of Study ST000436
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000336. The data can be accessed directly via it's Project DOI: 10.21228/M8NG6W This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000436 |
| Study Title | Metabolomic analysis of EE urine samples |
| Study Type | Untargeted metabolomics for disease biomarker |
| Study Summary | To conduct metabolomic analysis comparing 60 urine samples from the EE patients. |
| Institute | University of Minnesota |
| Department | Department of Food Science and Nutrition |
| Laboratory | Nutritional Metabolomics |
| Last Name | Chen |
| First Name | Chi |
| Address | 1334 Eckles Ave W, St Paul, MN 55108 |
| chichen@umn.edu | |
| Phone | 612-624-7704 |
| Submit Date | 2016-08-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML, raw(Waters) |
| Chear Study | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-12-31 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000336 |
| Project DOI: | doi: 10.21228/M8NG6W |
| Project Title: | Pilot study in UMN CHEAR untargeted resource |
| Project Summary: | This pilot study in the UMN CHEAR untargeted resource analyzed the urine samples from the EE patients. |
| Institute: | University of Minnesota |
| Department: | Department of Food Science and Nutrition |
| Laboratory: | Nutritional Metabolomics |
| Last Name: | Chen |
| First Name: | Chi |
| Address: | 1334 Eckles Ave W, St Paul, MN 55108 |
| Email: | chichen@umn.edu |
| Phone: | 612-624-7704 |
Subject:
| Subject ID: | SU000457 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Factor |
|---|---|---|
| SA021920 | C-L5U20-U | data1 |
| SA021921 | C-XU8T2-U | data1 |
| SA021922 | C-EQFC4-U | data1 |
| SA021923 | C-2VA08-U | data1 |
| SA021924 | C-S2A61-U | data1 |
| SA021925 | C-TPTM5-U | data1 |
| SA021926 | C-VUA04-U | data1 |
| SA021927 | C-2PN24-U | data1 |
| SA021928 | C-W34N5-U | data1 |
| SA021929 | C-636M9-U | data1 |
| SA021930 | C-P9HQ3-U | data1 |
| SA021931 | C-JSH03-U | data1 |
| SA021932 | C-WH2K8-U | data1 |
| SA021933 | C-EQSR8-U | data1 |
| SA021934 | C-FDB14-U | data1 |
| SA021935 | C-HXLY0-U | data1 |
| SA021936 | C-F0LB1-U | data1 |
| SA021937 | C-Y0XV6-U | data1 |
| SA021938 | C-R4A68-U | data1 |
| SA021939 | C-3CC98-U | data1 |
| SA021940 | C-2H0C5-U | data1 |
| SA021941 | C-QFLT2-U | data1 |
| SA021942 | C-BJ3E6-U | data1 |
| SA021943 | C-8JAD4-U | data1 |
| SA021944 | C-PT090-U | data1 |
| SA021945 | C-54M32-U | data1 |
| SA021946 | C-GRWN8-U | data1 |
| SA021947 | C-A09P4-U | data1 |
| SA021948 | C-LGBA7-U | data1 |
| SA021949 | C-JCS73-U | data1 |
| SA021950 | C-PWY28-U | data1 |
| SA021951 | C-90MV9-U | data1 |
| SA021952 | C-A9M21-U | data1 |
| SA021953 | C-D5GK7-U | data1 |
| SA021954 | C-SQGX2-U | data1 |
| SA021955 | C-5EA69-U | data1 |
| SA021956 | C-T05S2-U | data1 |
| SA021957 | C-51441-U | data1 |
| SA021958 | C-67LA4-U | data1 |
| SA021959 | C-LT9Y4-U | data1 |
| SA021960 | C-T0VM3-U | data1 |
| SA021961 | C-KUDE6-U | data1 |
| SA021962 | C-NAEG2-U | data1 |
| SA021963 | C-S7AH1-U | data1 |
| SA021964 | C-4CL23-U | data1 |
| SA021965 | C-836L9-U | data1 |
| SA021966 | C-MTN69-U | data1 |
| SA021967 | C-MGAJ7-U | data1 |
| SA021968 | C-KKX12-U | data1 |
| SA021969 | C-BFQS4-U | data1 |
| SA021970 | C-CQLC0-U | data1 |
| SA021971 | C-RYDY6-U | data1 |
| SA021972 | C-K4393-U | data1 |
| SA021973 | C-5YSX2-U | data1 |
| SA021974 | C-XM445-U | data1 |
| SA021975 | C-K0Y51-U | data1 |
| SA021976 | C-2PBR6-U | data1 |
| SA021977 | C-JEG62-U | data1 |
| SA021978 | C-2BTR9-U | data1 |
| SA021979 | C-QJX83-U | data1 |
| Showing results 1 to 60 of 60 |
Collection:
| Collection ID: | CO000451 |
| Collection Summary: | Urine samples were collected from the EE patients |
| Sample Type: | Urine |
Treatment:
| Treatment ID: | TR000471 |
| Treatment Summary: | No treatment was identified |
Sample Preparation:
| Sampleprep ID: | SP000464 |
| Sampleprep Summary: | Sixty human urine samples were thawed on ice. Aliquots of 50 uL urine samples were mixed with 50uL of 50% acetonitrile in water with 150uM d5-trptophan (internal standard). The mixture was vortexed at 3000rpm for 30s, and centrifuged at 21000g at 4 oC for 10 min. An aliquot of 100 uL supernatant was transferred to HPLC vials and analyzed by LC/MS. |
| Processing Method: | Extraction, vortex, centrifugation |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 50% acetonitrile in water with 150uM L-trptophan-indole-d5 as internal standard |
| Extract Storage: | - 20 C |
Chromatography:
| Chromatography ID: | CH000498 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters ACQUITY UPLC BEH C18 |
| Column Pressure: | 15000psi (Max) |
| Column Temperature: | 40 C |
| Flow Gradient: | 0-0.5min-0.5%B, 0.5-4min-gradient to 20%B, 4-8min-gradient to 95%B, 8-8.1min-gradient to 100%B, 8.1-9min-100%B, 9-9.1min gradient to 0.5%B, 9.1-10min-0.5%B |
| Flow Rate: | 0.5mL/min |
| Internal Standard: | L-trptophan-indoel-d5 |
| Retention Time: | 1.93min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Analytical Time: | 10min |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN000686 |
| Analysis Type: | MS |
| Instrument Name: | Waters Xevo-G2-S |
| Chromatography ID: | CH000498 |
| Num Factors: | 1 |
| Has Mz: | 1 |
| Has Rt: | 1 |
| Rt Units: | Minutes |
| Units: | peak area |
