Summary of Study ST000449
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000347. The data can be accessed directly via it's Project DOI: 10.21228/M8689X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000449 |
| Study Title | Molecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry |
| Study Summary | This study investigates the metabolism and antibiotic inhibition in Escherichia coli and Bacillus subtilis colonies. |
| Institute | George Washington University |
| Department | Chemistry |
| Laboratory | Vertes Research Lab |
| Last Name | Li |
| First Name | Hang |
| Address | 800 22nd St. NW, Suite 4000, Washington, DC 20052 |
| amy_li@gwmail.gwu.edu | |
| Phone | 202-994-6344 |
| Submit Date | 2016-08-09 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000347 |
| Project DOI: | doi: 10.21228/M8689X |
| Project Title: | Molecular Imaging of Growth, Metabolism, and Antibiotic Inhibition in Bacterial Colonies by Laser Ablation Electrospray Ionization Mass Spectrometry |
| Project Summary: | This study utilized laser ablation electrospray ionization mass spectrometry imaging to study the metabolism and antibiotic inhibition in bacterial colonies. |
| Institute: | The George Washington University |
| Department: | Chemistry |
| Laboratory: | Vertes Research Lab |
| Last Name: | Li |
| First Name: | Hang |
| Address: | 800 22nd St. NW, Suite 4000, Washington, DC 20052 |
| Email: | amy_li@gwmail.gwu.edu |
| Phone: | 202-994-6344 |
Subject:
| Subject ID: | SU000470 |
| Subject Type: | Microbial (Escherichia coli, bacillus subtilis) |
| Subject Species: | Bacillus subtilis |
| Taxonomy ID: | 1423 |
| Genotype Strain: | ATCC12435, ATCC6051 |
| Species Group: | Microorganism |
Factors:
Subject type: Microbial (Escherichia coli, bacillus subtilis); Subject species: Bacillus subtilis (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment |
|---|---|---|
| SA022780 | EC-1 | Blank |
| SA022781 | BS-1 | Blank |
| SA022782 | ECBS-1 | Streptomycin |
| SA022783 | BS-2 | Streptomycin-penicillin |
| Showing results 1 to 4 of 4 |
Collection:
| Collection ID: | CO000464 |
| Collection Summary: | All the sample were analyzed using laser ablation electrospray ionization (LAESI) MS or MSI. |
| Sample Type: | Bacterial cells |
Treatment:
| Treatment ID: | TR000484 |
| Treatment Summary: | In the antibiotic susceptibility testing, the bacterial colonies were treated with streptomycin or streptomycin-penicillin. |
Sample Preparation:
| Sampleprep ID: | SP000477 |
| Sampleprep Summary: | The E. coli and B. subtilis colonies were inoculated and incubated following the standard protocol. The antibiotic inhibition tests were performed according to the conventional Kirby-Bauer antibiotic testing. |
Combined analysis:
| Analysis ID | AN000703 | AN000704 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | None (Direct infusion) | None (Direct infusion) |
| Chromatography system | Synapt G2S | Synapt G2S |
| Column | None | None |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Waters Synapt G2 S QTOF | Waters Synapt G2 S QTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | m/z | m/z |
Chromatography:
| Chromatography ID: | CH000509 |
| Chromatography Summary: | No chromatography was used for this study |
| Instrument Name: | Synapt G2S |
| Column Name: | None |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS000625 |
| Analysis ID: | AN000703 |
| Instrument Name: | Waters Synapt G2 S QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| MS ID: | MS000626 |
| Analysis ID: | AN000704 |
| Instrument Name: | Waters Synapt G2 S QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
