Summary of Study ST000461

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000354. The data can be accessed directly via it's Project DOI: 10.21228/M8930Q This work is supported by NIH grant, U2C- DK119886.

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Study IDST000461
Study TitleEffect of Odc knockout on amino acid homeostasis of Eu-Myc Lymphomas
Study TypeLymphomas isolated from Eu-Myc;CD19-Cre;Odc fl/fl mice
Study SummaryAmino acid levels analysis of lymphomas isolated from Eu-Myc;CD19-Cre;Odc fl/fl mic and their wild-type counterparts Eu-Myc;CD19-Cre;Odc +/+
Institute
University of Florida
DepartmentSECIM
LaboratoryJohn Cleveland
Last NameFernandez
First NameMario
Address12902 Magnolia Drive, MRC 4-West
Emailmario.fernandez@moffitt.org
Phone813-745-5140
Submit Date2016-09-08
Num Groups4
Total Subjects12
Study CommentsLymphomas isolated from Eu-Myc;CD19-Cre;Odc fl/fl mice in triplicate
Analysis Type DetailLC-MS
Release Date2020-07-08
Release Version1
Mario Fernandez Mario Fernandez
https://dx.doi.org/10.21228/M8930Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000354
Project DOI:doi: 10.21228/M8930Q
Project Title:The effect of BCAT1 knockdown on amino acid homeostasis
Project Type:Amino acid steady-state level analysis
Project Summary:Raji cells, a Burkitt lymphoma, expressing a Dox-inducible short hairpin targeting BCAT1 were collected in triplicate at 0, 48, 96, and 144 hours.
Institute:H. Lee Moffitt Cancer Center
Department:Tumor Biology
Laboratory:John Cleveland
Last Name:Fernandez
First Name:Mario
Address:12902 Magnolia Drive, MRC 4-West
Email:mario.fernandez@moffitt.org
Phone:813-745-5140
Funding Source:5R01CA154739

Subject:

Subject ID:SU000482
Subject Type:Cells
Subject Species:Mus musculus
Taxonomy ID:10090
Cell Strain Details:BIT
Subject Comments:See study design section for cell number per sample
Cell Primary Immortalized:Immortalized
Species Group:Mammals

Factors:

Subject type: Cells; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Genotype
SA023256BIT_001Odc +/+
SA023257BIT_006Odc +/+
SA023258BIT_005Odc +/+
SA023259BIT_002Odc +/+
SA023260BIT_003Odc +/+
SA023261BIT_004Odc +/+
SA023262BIT_012Odc -/-
SA023263BIT_011Odc -/-
SA023264BIT_007Odc -/-
SA023265BIT_008Odc -/-
SA023266BIT_009Odc -/-
SA023267BIT_010Odc -/-
Showing results 1 to 12 of 12

Collection:

Collection ID:CO000476
Collection Summary:Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C.
Sample Type:Cell
Collection Method:Pellet
Collection Location:Lab
Collection Time:Collected during log phase
Storage Conditions:Pellet at -80C
Storage Vials:1.5 mL microfuge tube
Tissue Cell Identification:BIT

Treatment:

Treatment ID:TR000496
Treatment Summary:Cells were not treated. Cells were collected during log phase
Cell Growth Container:6-well plate

Sample Preparation:

Sampleprep ID:SP000489
Sampleprep Summary:Amino acid
Sampleprep Protocol Filename:SB_AA_Assay.pdf

Chromatography:

Chromatography ID:CH000519
Chromatography Summary:Amino Acid
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN000722
Laboratory Name:Sanford Burnham
Analysis Type:MS
Instrument Name:Agilent 6490
Chromatography ID:CH000519
Num Factors:2
Num Metabolites:17
Rt Units:Minutes
Units:nmol/mg protein
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