Summary of study ST000466

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000358. The data can be accessed directly via it's Project DOI: 10.21228/M8S302 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000466
Study TitleStreptococcus mutans organic acids/amino acid profiles, effect of oxidative stress; pilot
Study TypeAmino acid/organic acid profiles of wild-type and mutant strain in response to H2O2 treatment over time.
Study SummaryS. mutans UA159 and ΔspxA1 will be grown in BHI to OD600 = 0.4. Each culture will be split in to 4 samples. For the first sample, 3E10 cells will immediately be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. The remaining three samples will be treated with H2O2 to 0.5 mM, and the harvesting process repeated after 15, 30 and 60 minutes.
Institute
University of Florida
DepartmentSECIM
LaboratoryLemos-Abranches
Last NameHardin
First NameEmily
Address1395 Center Drive, PO Box 100424, Gainesville, FL 32610
Emailehardin@ufl.edu
Phone352-273-8844
Submit Date2016-09-08
Num Groups6
Total Subjects6
Raw Data AvailableYes
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Emily Hardin Emily Hardin
https://dx.doi.org/10.21228/M8S302
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000358
Project DOI:doi: 10.21228/M8S302
Project Title:Streptococcus mutans Amino Acid/Organic Acid Profile
Project Summary:Determination of metabolite profiles of S. mutans strains UA159 (WT) and ΔspxA1 in response to oxidative stress
Institute:University of Florida
Department:Oral Biology
Laboratory:Lemos-Abranches
Last Name:Lemos
First Name:Jose
Address:1395 Center Drive, PO Box 100424 Gainesville, FL 32610
Email:jlemos@dental.ufl.edu
Phone:352-273-8843
Funding Source:UF start-up funds
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