Summary of study ST000475

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000362. The data can be accessed directly via it's Project DOI: 10.21228/M8831S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000475
Study TitleMetabolomic testing of a putative MDH inhibitor (global)
Study Typedrug treatment (single dose and timepoint)
Study SummaryHL-60 cells will be treated for 15hr with a putative MDH inhibitor or vehicle (n=5 per group)
Institute
University of Florida
DepartmentSECIM
LaboratoryCase Western University
Last NameWald
First NameDavid
Address2103 Cornell Rd WRB3-530 Cleveland, OH 44106
Emaildnw@case.edu
Phone2163685668
Submit Date2016-09-12
Num Groups2
Total Subjects20
Study CommentsTotal subjects: 10 for global testing and 10 for targeted testing
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
David Wald David Wald
https://dx.doi.org/10.21228/M8831S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000362
Project DOI:doi: 10.21228/M8831S
Project Title:Metabolomic testing of a putative MDH inhibitor
Project Summary:Testing for metabolomic changes after treatment of leukemia cells with a putative MDH inhibitor
Institute:Case Western Reserve University
Department:Pathology
Laboratory:Foundation and Institutional funds
Last Name:Wald
First Name:David
Address:2103 Cornell Rd WRB3-530 Cleveland, OH 44106
Email:dnw@case.edu
Phone:NA

Subject:

Subject ID:SU000496
Subject Type:Human leukemia cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Cell Strain Details:HL-60
Subject Comments:immortalized
Cell Counts:10 million per sample
Species Group:Human

Factors:

Subject type: Human leukemia cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA024338ut1-
SA024339ut5-
SA024340ut4-
SA024341ut2-
SA024342ut3-
SA024343tx5MDH inhib
SA024344tx4MDH inhib
SA024345tx1MDH inhib
SA024346tx2MDH inhib
SA024347tx3MDH inhib
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000490
Collection Summary:Cells were centrifuged to remove growth media/drug and either flash frozen or washed 1x in PBS. The cells were washed for the targeted assay but not for the global assay.
Sample Type:cell
Storage Conditions:flash frozen
Tissue Cell Quantity Taken:10 million per sample

Treatment:

Treatment ID:TR000510
Treatment Summary:Cells were treated for 15hr with 1 micromolar of drug or vehicle
Cell Growth Container:flasks
Cell Growth Rate:~24hr per division
Cell Media:RPMI with 10% FBS
Cell Envir Cond:5% CO2 incubator
Cell Media Lastchanged:15 hr

Sample Preparation:

Sampleprep ID:SP000503
Sampleprep Summary:Amino Acid|Global
Sampleprep Protocol Filename:SB_OA_Assay.pdf
Appendix_C_Protein_DNA_quant.pdf
Sample Spiking:Appendix A - Internal Standard Prep GLCMS.pdf

Combined analysis:

Analysis ID AN000739 AN000740
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak area Peak area

Chromatography:

Chromatography ID:CH000532
Chromatography Summary:Global
Methods Filename:Consolidated_Cellular_Metabolomics_SOP.pdf
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000656
Analysis ID:AN000739
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000657
Analysis ID:AN000740
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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