Summary of study ST000478

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000364. The data can be accessed directly via it's Project DOI: 10.21228/M80K61 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000478
Study TitleNMR based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation
Study SummaryTissue extracts from ionizing readiation exposed mouse liver and controls were compared via NMR based metabolomic analysis
Institute
Pacific Northwest National Laboratory
DepartmentFundamental & Computational Sciences
Last NameHu
First NameJianzhi
AddressPacific Northwest National Laboratory, Richland, WA 99352
EmailJianzhi.Hu@pnnl.gov
Phone+1 (509) 371-6544
Submit Date2016-08-11
Raw Data AvailableYes
Raw Data File Type(s).fid, log, procpar,text
Analysis Type DetailNMR
Release Date2017-07-10
Release Version1
Jianzhi Hu Jianzhi Hu
https://dx.doi.org/10.21228/M80K61
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000364
Project DOI:doi: 10.21228/M80K61
Project Title:NMR based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation
Project Summary:Tissue extracts from ionizing readiation exposed mouse liver and controls were compared via NMR based metabolomic analysis
Institute:Pacific Northwest National Laboratory
Department:Fundamental & Computational Sciences
Last Name:Hu
First Name:Jianzhi
Address:Pacific Northwest National Laboratory, Richland, WA 99352
Email:Jianzhi.Hu@pnnl.gov
Phone:+1 (509) 371-6544

Subject:

Subject ID:SU000499
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id days post exposure to radiation radiation dose radiation source
SA024487G31511 3.0 gamma
SA024488G31111 3.0 gamma
SA024489G31411 3.0 gamma
SA024490G31311 3.0 gamma
SA024491G31211 3.0 gamma
SA024492P31111 3.0 proton
SA024493P31211 3.0 proton
SA024494P31511 3.0 proton
SA024495P31411 3.0 proton
SA024496P31311 3.0 proton
SA024483G01311 - sham
SA024484G01411 - sham
SA024485G01211 - sham
SA024486G01111 - sham
SA024501G3444 3.0 gamma
SA024502G3424 3.0 gamma
SA024503G3434 3.0 gamma
SA024504G3454 3.0 gamma
SA024505G3414 3.0 gamma
SA024506G7434 7.8 gamma
SA024507G7444 7.8 gamma
SA024508G7424 7.8 gamma
SA024509G7414 7.8 gamma
SA024497G0424 - sham
SA024498G0434 - sham
SA024499G0444 - sham
SA024500G0414 - sham
Showing results 1 to 27 of 27

Collection:

Collection ID:CO000493
Collection Summary:Livers were dissected and snap-frozen at -80°C before NMR spectroscopy and morphology analysis.
Sample Type:Liver

Treatment:

Treatment ID:TR000513
Treatment Summary:A total of 27 seven-week-old C57BL/6 female mice randomly into 6 groups, and then whole body being exposed to either gamma or proton radiation. The individually housed animals were fed a standard diet and maintained in an environment controlled facility with an ambient temperature of 22-25°C and 45% relative humidity on 12 h light-dark cycle. Mice were exposed to whole body gamma radiation using a high-activity source (1250 keV 60Co) with the linear energy transfer (LET) associated with these fields in the range of 0.2-2 keV/μm. The mice were isolated in the corner of their polymer cages and placed a minimum of 100 cm from the collimated 6000 Ci (222 TBq) 60Co source, and then irradiation to the proposed dosage, respectively. The result of absorbed dose rate at a depth of approximately 600 mg/cm2 were ~0.83 Gy/min relative to tissue. After irradiation the isolation barrier was removed and mice were transferred to Animal Facility.Mice placed into aerated polystyrene boxes immediately before exposure to the entrance region of proton at the BNL Alternating Gradient Synchrotron (AGS) (1055 MeV/nucleon at the target, with a track-averaged LET =148.2 keV/μm at target). Doses of 0 and 3.0 Gy were delivered to a maximum of four mice at ~0.83 Gy/min in a single fraction. All procedures (i.e., placement in aerated boxes and subsequent tissue harvesting) for the control mice were identical to those of irradiated animals, except that they received no radiation.

Sample Preparation:

Sampleprep ID:SP000506
Sampleprep Summary:"Step 1: After randomization of the samples, every preweighted intact frozen liver tissue was homogenized by a Tissue Tearsor (Model 985-370, BioSpec Product, Inc.) in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements."

Analysis:

Analysis ID:AN000744
Analysis Type:NMR
Num Factors:6
Num Metabolites:72
Units:µM/mg

NMR:

NMR ID:NM000083
Analysis ID:AN000744
Instrument Name:Varian INOVA 800
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:800 MHz
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