Summary of Study ST000505
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000505 |
| Study Title | Timecourse of Amino Acid Metabolites in LNCaP Cell pellets |
| Study Type | timecourse |
| Study Summary | Timecourse of Amino Acid Metabolites in LNCaP Cell pellets |
| Institute | Mayo Clinic |
| Laboratory | Mayo Metabolomics Core |
| Last Name | Frigo |
| First Name | Daniel |
| Address | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| frigo@uh.edu | |
| Phone | 832-842-8824 |
| Submit Date | 2016-10-21 |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-10-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000371 |
| Project DOI: | doi: 10.21228/M83C78 |
| Project Title: | Metabolic Dissection of Aberrant Glutamine Metabolism in Prostate Cancer |
| Project Type: | MS targeted analysis |
| Project Summary: | In this project, we propose to define the specific role(s) of three crucial oncogenic signaling cascades (androgen receptor, Myc and AMPK) in regulating glutamine metabolism in prostate cancer using 13C-labeled glutamine with mass spectrometry-based metabolic flux analyses. |
| Institute: | Mayo Clinic |
| Laboratory: | Mayo Clinic Metabolomics Resource Core |
| Last Name: | Frigo |
| First Name: | Daniel |
| Address: | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| Email: | frigo@uh.edu |
| Phone: | 832-842-8824 |
Subject:
| Subject ID: | SU000527 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Treatment Factor | 13C media incubation time |
|---|---|---|---|
| SA026266 | ms5459-6 | treated-100 pM R1881 | 24 hours |
| SA026267 | ms5459-2 | treated-100 pM R1881 | 24 hours |
| SA026268 | ms5459-13 | treated-100 pM R1881 | 72 hours |
| SA026269 | ms5459-9 | treated-100 pM R1881 | 72 hours |
| SA026262 | ms5459-7 | treated-10 nM R1881 | 24 hours |
| SA026263 | ms5459-4 | treated-10 nM R1881 | 24 hours |
| SA026264 | ms5459-11 | treated-10 nM R1881 | 72 hours |
| SA026265 | ms5459-14 | treated-10 nM R1881 | 72 hours |
| SA026260 | ms5459-3 | treated-1 nM R1881 | 24 hours |
| SA026261 | ms5459-10 | treated-1 nM R1881 | 72 hours |
| SA026270 | ms5459-1 | treated-vehicle (ethanol) | 24 hours |
| SA026271 | ms5459-5 | treated-vehicle (ethanol) | 24 hours |
| SA026272 | ms5459-8 | treated-vehicle (ethanol) | 72 hours |
| SA026273 | ms5459-12 | treated-vehicle (ethanol) | 72 hours |
| Showing results 1 to 14 of 14 |
Collection:
| Collection ID: | CO000521 |
| Collection Summary: | LNCaP cell media |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000541 |
| Treatment Summary: | LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours. |
Sample Preparation:
| Sampleprep ID: | SP000534 |
| Sampleprep Summary: | Concentration of Amino Acids metabolites in cell media |
Chromatography:
| Chromatography ID: | CH000554 |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN000774 |
| Analysis Type: | MS |
| Instrument Name: | Thermo TSQ Quantum Ultra |
| Chromatography ID: | CH000554 |
| Num Factors: | 8 |
| Num Metabolites: | 42 |
| Units: | micromolar |
