Summary of Study ST000507
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000371. The data can be accessed directly via it's Project DOI: 10.21228/M83C78 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000507 |
| Study Title | Timecourse of TCA isotopomers in LNCaP Cells |
| Study Summary | Timecourse of TCA isotopomers in LNCaP Cells |
| Institute | Mayo Clinic |
| Laboratory | Mayo Clinic Metabolomics Resource Core |
| Last Name | Frigo |
| First Name | Daniel |
| Address | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| frigo@uh.edu | |
| Phone | 832-842-8824 |
| Submit Date | 2016-10-28 |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000371 |
| Project DOI: | doi: 10.21228/M83C78 |
| Project Title: | Metabolic Dissection of Aberrant Glutamine Metabolism in Prostate Cancer |
| Project Type: | MS targeted analysis |
| Project Summary: | In this project, we propose to define the specific role(s) of three crucial oncogenic signaling cascades (androgen receptor, Myc and AMPK) in regulating glutamine metabolism in prostate cancer using 13C-labeled glutamine with mass spectrometry-based metabolic flux analyses. |
| Institute: | Mayo Clinic |
| Laboratory: | Mayo Clinic Metabolomics Resource Core |
| Last Name: | Frigo |
| First Name: | Daniel |
| Address: | Department of Biology and Biochemistry, University of Houston, Houston, Texas 77204-5001 |
| Email: | frigo@uh.edu |
| Phone: | 832-842-8824 |
Subject:
| Subject ID: | SU000529 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Time (incubation) |
|---|---|---|
| SA026288 | DF-8 | 24 hours |
| SA026289 | DF-1 | 24 hours |
| SA026290 | DF-7 | 24 hours |
| SA026291 | DF-6 | 24 hours |
| SA026292 | DF-2 | 24 hours |
| SA026293 | DF-4 | 24 hours |
| SA026294 | DF-3 | 24 hours |
| SA026295 | DF-5 | 24 hours |
| SA026296 | DF-15 | 72 hours |
| SA026297 | DF-16 | 72 hours |
| SA026298 | DF-14 | 72 hours |
| SA026299 | DF-9 | 72 hours |
| SA026300 | DF-10 | 72 hours |
| SA026301 | DF-11 | 72 hours |
| SA026302 | DF-12 | 72 hours |
| SA026303 | DF-13 | 72 hours |
| Showing results 1 to 16 of 16 |
Collection:
| Collection ID: | CO000523 |
| Collection Summary: | LNCaP cell models |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000543 |
| Treatment Summary: | LNCaP cells that were untreated. |
Sample Preparation:
| Sampleprep ID: | SP000536 |
| Sampleprep Summary: | TCA isotopomers |
| Sampleprep Protocol Comments: | cells incubated in U-13C5-glutamine containing media |
Combined analysis:
| Analysis ID | AN000776 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | % enrichment |
Chromatography:
| Chromatography ID: | CH000556 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent HP5-MS (30m × 0.25mm, 0.25 um) |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000683 |
| Analysis ID: | AN000776 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
