Summary of study ST000509

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000382. The data can be accessed directly via it's Project DOI: 10.21228/M8P597 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000509
Study TitleMetabolic changes to maternal rat liver tissue during and post-pregnancy (part I)
Study SummaryAssessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute
University of Colorado, Denver
Last NameNemkov
First NameTravis
Address12801 E 17th Avenue RC-1 South, Mail stop 8101
Emailtravis.nemkov@ucdenver.edu
Phone303-724-5798
Submit Date2016-12-04
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2017-01-30
Release Version1
Travis Nemkov Travis Nemkov
https://dx.doi.org/10.21228/M8P597
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000382
Project DOI:doi: 10.21228/M8P597
Project Title:Metabolic changes to maternal rat liver tissue during and post-pregnancy.
Project Type:global MS analysis
Project Summary:Assessing metabolic changes to maternal rat liver tissue during and post-pregnancy. Liver tissue was harvested post-mortem from rats or mice without pregnancy (Nulliparous - NP), time course during pregnancy in days (P2-4, P11-13m P18-20), lactation day 10 (LD10), in during involution of the liver (I2, I4, I6, I8, I10) and 4-weeks regression after preganancy (R4).
Institute:University of Colorado, Denver
Last Name:Nemkov
First Name:Travis
Address:12801 E 17th Avenue, RC-1 South, Rm 9403G, Aurora, CO, 80045, USA
Email:travis.nemkov@ucdenver.edu
Phone:303-724-5798

Subject:

Subject ID:SU000531
Subject Type:Animal
Subject Species:Rattus norvegicus
Taxonomy ID:10116
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id time_point
SA026500I10_60I10
SA026501I10_70I10
SA026502I10_64I10
SA026503I10_73I10
SA026504I2_90I2
SA026505I2_93I2
SA026506I2_104I2
SA026507I2_79I2
SA026508I2_92I2
SA026509I4_72I4
SA026510I4_101I4
SA026511I4_71I4
SA026512I4_96I4
SA026513I6_81I6
SA026514I6_94I6
SA026515I6_95I6
SA026516I6_77I6
SA026517I6_66I6
SA026518I8_75I8
SA026519I8_98I8
SA026520I8_97I8
SA026521I8_63I8
SA026522I8_67I8
SA026523I8_74I8
SA026524LD10_99LD10
SA026525LD10_9LD10
SA026526LD10_22LD10
SA026527LD10_31LD10
SA026528LD10_103LD10
SA026529NP_91NP
SA026530NP_58NP
SA026531NP_57NP
SA026532NP_102NP
SA026533NP_3NP
SA026534NP_2NP
SA026535P11-13_25P11-13
SA026536P11-13_17P11-13
SA026537P11-13_16P11-13
SA026538P11-13_18P11-13
SA026539P18-20_23P18-20
SA026540P18-20_76P18-20
SA026541P18-20_61P18-20
SA026542P18-20_69P18-20
SA026543P18-20_7P18-20
SA026544P18-20_15P18-20
SA026545P2-4_13P2-4
SA026546P2-4_12P2-4
SA026547P2-4_11P2-4
SA026548P2-4_14P2-4
SA026549P2-4_10P2-4
SA026550R4_83R4
SA026551R4_62R4
SA026552R4_30R4
SA026553R4_24R4
SA026554R4_26R4
SA026555R4_32R4
Showing results 1 to 56 of 56

Collection:

Collection ID:CO000525
Collection Summary:tissue was harvested, flash frozen in LiN2, milled with LiN2, and weighed on dry ice for metabolomics analysis
Sample Type:Liver

Treatment:

Treatment ID:TR000545
Treatment Summary:Tissues were harvested at the appropriate time point during or post-pregnancy

Sample Preparation:

Sampleprep ID:SP000538
Sampleprep Summary:Frozen and milled liver tissue was suspended to 10 mg/ml in an extraction solution containing methanol/acetonitrile/water at a ratio of 5:3:2 (v/v/v). Suspensions were vortexed at 4C for 30 minutes and centrifuged at 10,000 g for 10 minutes, 4C. Supernatants were isolated and analyzed by LC-MS.

Combined analysis:

Analysis ID AN000780 AN000781
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Kinetex XB-C18 Kinetex XB-C18
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units arbitrary units arbitrary units

Chromatography:

Chromatography ID:CH000559
Instrument Name:Thermo Vanquish
Column Name:Kinetex XB-C18
Chromatography Type:Reversed phase

MS:

MS ID:MS000687
Analysis ID:AN000780
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000688
Analysis ID:AN000781
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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