Summary of study ST000541

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000396. The data can be accessed directly via it's Project DOI: 10.21228/M8VP5F This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000541
Study TitleCanine Diabetes - Preliminary Evaluation of Testing Methods
Study TypeSingle time point blood collection
Study SummaryBlood was collected from diabetic dogs and healthy control dogs. Diabetic dogs and control dogs were breed matched where possible. Timing of blood collection after a meal was matched as best as possible. Serum was frozen at -80C.
Institute
University of Florida
DepartmentSECIM
Last NameO'Kell
First NameAllison
Address2015 SW 16th Ave
Emailaokell@ufl.edu
Phone352-392-2235
Submit Date2017-01-10
Num Groups2
Total Subjects24
PublicationsO'Kell AL1, Garrett TJ2, et al. "Untargeted metabolomic analysis in naturally occurring canine diabetes mellitus identifies similarities to human Type 1 Diabetes." Sci Rep. 2017 Aug 25;7(1):9467. doi: 10.1038/s41598-017-09908-5.
Raw Data AvailableYes
Raw Data File Type(s).mzML
Analysis Type DetailLC-MS
Release Date2018-04-10
Release Version1
Allison O'Kell Allison O'Kell
https://dx.doi.org/10.21228/M8VP5F
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000396
Project DOI:doi: 10.21228/M8VP5F
Project Title:Utility of canine diabetes as a model for human type 1 diabetes
Project Summary:We are examining the etiopathogenesis of canine diabetes to determine the relevance of these animals as a naturally occurring model of human type 1 diabetes. We will undertake phenotypic, metabolic, and immunologic investigations in diabetic dogs to better characterize their diabetes and attempt to identify potential biomarkers of a pre-diabetic state.
Institute:University of Florida
Department:Diabetes Institute (Wasserfall/Atkinson)/College of Veterinary Medicine (O'Kell)
Laboratory:Atkinson Lab
Last Name:O'Kell
First Name:Allison
Address:2015 SW 16th Ave.
Email:aokell@ufl.edu
Phone:352-392-2235

Subject:

Subject ID:SU000563
Subject Type:Animal
Subject Species:Canis lupus familiaris
Taxonomy ID:9615
Age Or Age Range:1 year - 15 years
Weight Or Weight Range:5 kg - 40 kg
Gender:Male Neutered or Female Spayed
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Canis lupus familiaris (Factor headings shown in green)

mb_sample_id local_sample_id Health Status Gender Fasting Status Breed Size (S/M/L)
SA027995213269Diabetic Neutered male Fasted Labrador retriever Large
SA027996313390Diabetic Neutered male Fasted Miniature schnauzer Small
SA027997325103Diabetic Neutered male Fasted Mixed Large
SA027998216422Diabetic Neutered male Fed Dachshund Small
SA027999309443Diabetic Neutered male Fed Mixed Medium
SA028000321588Diabetic Spayed female Fasted Dachshund Small
SA028001325942Diabetic Spayed female Fasted Dachshund Small
SA028002213267Diabetic Spayed female Fasted Labrador retriever Large
SA028003279436Diabetic Spayed female Fed Labrador retriever Large
SA028004325643Diabetic Spayed female Fed Labrador retriever Large
SA028005269031Diabetic Spayed female Fed Mixed Small
SA028006323294Diabetic Spayed female Fed Shih Tzu Small
SA028007290374Healthy Neutered male Fasted Flat coated retriever Large
SA028008255293Healthy Neutered male Fasted Labrador retriever Large
SA028009223169Healthy Neutered male Fed Dachshund Small
SA028010290195Healthy Neutered male Fed Labrador retriever Large
SA028011325932Healthy Neutered male Fed Mixed Medium
SA028012296735Healthy Neutered male Fed Mixed Small
SA028013326077Healthy Neutered male Fed Shih Tzu Small
SA028014325866Healthy Spayed female Fasted Dachshund Small
SA028015288261Healthy Spayed female Fasted Dachshund Small
SA028016228415Healthy Spayed female Fasted Labrador retriever Large
SA028017295029Healthy Spayed female Fasted Miniature schnauzer Small
SA028018287971Healthy Spayed female Fed Labrador retriever Large
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000557
Collection Summary:Blood collected by peripheral venipuncture into red top tube (containing clot activator). Blood was allowed to clot, then centrifuged at 2000 g x 7 minutes between 20-30 minutes after sample collection. Serum was frozen immediately after separation (always within 1 hour of collection) at -80 C.
Sample Type:Blood (serum)
Collection Method:Peripheral venipuncture
Collection Location:Jugular, saphenous, or cephalic vein
Collection Frequency:Once
Storage Conditions:Room temperature until centrifugation. Then -80 C immediately after separation from red cells. Serum was thawed once on ice, 100 uL aliquots taken, and aliquots re-frozen (at -80C) until use for metabolomics.
Collection Vials:Red top with clot activator
Storage Vials:Cryovials (polypropylene with silicone gasket - Fisher Brand)
Collection Tube Temp:Room temperature
Blood Serum Or Plasma:Serum

Treatment:

Treatment ID:TR000577
Treatment Summary:None

Sample Preparation:

Sampleprep ID:SP000570
Sampleprep Summary:See protocol
Sampleprep Protocol Filename:Metabolomics_LCMSProtocol_plasma.pdf

Combined analysis:

Analysis ID AN000822 AN000823
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific Q Exactive Thermo Scientific Q Exactive
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH000587
Chromatography Summary:see protocol
Instrument Name:Thermo Scientific Q Exactive
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000723
Analysis ID:AN000822
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000724
Analysis ID:AN000823
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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