Summary of study ST000554

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000406. The data can be accessed directly via it's Project DOI: 10.21228/M8K89B This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download all metabolite data  |  Perform analysis on untargeted data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data (Contains raw data)
Study IDST000554
Study TitleMetabolomics of aged iPSC
Study Typebaseline
Study SummaryThe aim of the study is to perform global metabolomics of aged iPSC.
Institute
University of Florida
DepartmentSECIM
LaboratoryMemorial Sloan Kettering Institute
Last NameDogan
First NameYildirim
Address417 E 68th St. New York NY 10021
Emaildogany@mskcc.org
Phone646-888-3578
Submit Date2017-02-01
Num Groups7
Total Subjects16
Study CommentsGroups: ESC / YiPSC / AiPSC / AiPSC_glut3 / AiPSC_ZSCAN10/ YiPSC_DCA/YiPSC_Citr
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Yildirim Dogan Yildirim Dogan
https://dx.doi.org/10.21228/M8K89B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000406
Project DOI:doi: 10.21228/M8K89B
Project Title:Metabolomics of aged iPSC
Project Summary:The aim of the study is to perform global metabolomics of aged iPSC.
Institute:Memorial Sloan Kettering Institute
Department:Cancer Biology
Last Name:Kim
First Name:Kitai
Address:417 E 68th St. New York NY 10021
Email:kimk@mskcc.org
Phone:646-888-3578

Subject:

Subject ID:SU000576
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Age Or Age Range:B6/CBA
Cell Primary Immortalized:B6/CBA
Cell Passage Number:Cultured primary cells
Cell Counts:16-18
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA028733kim_YiPSC_DCA_115mM Disodium acetate
SA028734kim_YiPSC_DCA_145mM Disodium acetate
SA028735kim_YiPSC_DCA_135mM Disodium acetate
SA028736kim_YiPSC_DCA_45mM Disodium acetate
SA028737kim_YiPSC_Citr_256mM Sodium Citrate
SA028738kim_YiPSC_Citr_396mM Sodium Citrate
SA028739kim_AiPSC_ZSCAN10_7Untreated
SA028740kim_AiPSC_ZSCAN10_19Untreated
SA028741kim_YiPSC_1-8Untreated
SA028742kim_YiPSC_1-3Untreated
SA028743kim_ESC-HUntreated
SA028744kim_AiPSC_16Untreated
SA028745kim_AiPSC_19Untreated
SA028746kim_AiPSC_glut3_gcn4Untreated
SA028747kim_AiPSC_glut3_gcn3Untreated
SA028748kim_ESC-EUntreated
Showing results 1 to 16 of 16

Collection:

Collection ID:CO000570
Collection Summary:none
Collection Protocol Comments:Cells were scraped off on ice in pbs
Sample Type:Cultured primary cells
Collection Location:Cold Room
Collection Frequency:two plates at a time
Collection Duration:15 mins
Volumeoramount Collected:In 5ml cold PBS
Storage Conditions:N2 snap frozen, stored at -80
Collection Vials:Falcon tube and eppendorf tube
Storage Vials:Eppendorf tube
Collection Tube Temp:<4C
Additives:None
Tissue Cell Quantity Taken:5-10mill

Treatment:

Treatment ID:TR000590
Treatment Summary:No treatment
Plant Humidity:-

Sample Preparation:

Sampleprep ID:SP000583
Sampleprep Summary:None
Sampleprep Protocol Filename:Metabolomics_Global_LCMS.pdf

Combined analysis:

Analysis ID AN000849 AN000850
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system None None
Column None None
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000606
Methods Filename:Metabolomics_Global_LCMS.pdf
Instrument Name:None
Column Name:None
Chromatography Type:Reversed phase

MS:

MS ID:MS000750
Analysis ID:AN000849
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI
Ion Mode:POSITIVE
  
MS ID:MS000751
Analysis ID:AN000850
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:HESI
Ion Mode:NEGATIVE
  logo