Summary of study ST000564

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000232. The data can be accessed directly via it's Project DOI: 10.21228/M8P01G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000564
Study Title2014 Biotron Experiment Metabolites (part II)
Study TypeTime course
Study SummaryMaize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.
Institute
University of Florida
DepartmentSECIM
LaboratoryStewart
Last NameStewart
First NameJon
Address102 Leigh Hall, University of Florida
Emailjds2@chem.ufl.edu
Phone352-846-0743
Num Groups1
Total Subjects6
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Jon Stewart Jon Stewart
https://dx.doi.org/10.21228/M8P01G
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000232
Project DOI:doi: 10.21228/M8P01G
Project Title:Climate Change and Starch Yield in Maize Part 2
Project Type:MS study
Project Summary:Maize plants were grown under three different temperature regimes: 1) normal day / normal night; 2) hot day / normal night; 3) hot day / hot night. Kernels from developing ears were taken 14, 16, 18, 22, 26 and 40 days after pollination.
Institute:University of Florida
Department:Chemistry
Laboratory:Stewart
Last Name:Stewart
First Name:Jon
Address:126 Sisler Hall
Email:jds2@chem.ufl.edu
Phone:846-0743
Funding Source:USDA

Subject:

Subject ID:SU000586
Subject Type:Plant
Subject Species:Zea mays
Taxonomy ID:4577
Species Group:Plant

Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Time
SA029060Control 14 DAP14 days after pollination
SA029061Control 16 DAP16 days after pollination
SA029062Control 18 DAP18 days after pollination
SA029063Control 22 DAP22 days after pollination
SA029064Control 26 DAP26 days after pollination
SA029065Control 40 DAP40 days after pollination
Showing results 1 to 6 of 6

Collection:

Collection ID:CO000580
Collection Summary:None
Sample Type:Maize kernel
Collection Method:Manual removal from ear
Collection Location:University of Wisconsin Biotron
Storage Conditions:Frozen at -80C

Treatment:

Treatment ID:TR000600
Treatment Summary:None
Plant Growth Location:Wisconsin Biotron Facility
Plant Temp:28 C day / 17 C night
Plant Metab Quench Method:Frozen at -80C

Sample Preparation:

Sampleprep ID:SP000593
Sampleprep Summary:None
Sampleprep Protocol Filename:Maize_Tissue_sample_prep.pdf

Combined analysis:

Analysis ID AN000866 AN000867
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units peak height peak height

Chromatography:

Chromatography ID:CH000616
Chromatography Summary:None
Instrument Name:Thermo Scientific-Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000767
Analysis ID:AN000866
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000768
Analysis ID:AN000867
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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