Summary of Study ST000572
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000420. The data can be accessed directly via it's Project DOI: 10.21228/M8RS3S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000572 |
| Study Title | Metabolomics of Shark Bay stromatolites |
| Study Type | comparison of two microbial mat types |
| Study Summary | microbial mats were collected from Shark Bay, Western Australia and we want to compare two mat types from this site. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Jamie foster |
| Last Name | Foster |
| First Name | Jamie |
| Address | Space Life Science Lab 505 Odyssey Way, merritt Island, FL 32953 |
| jfoster@ufl.edu | |
| Phone | 321-525-1047 |
| Submit Date | 2017-01-11 |
| Num Groups | 3 |
| Total Subjects | 30 |
| Study Comments | 3 groups - three different microbial mat types 30 samples - 10 samples per group |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-01-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000420 |
| Project DOI: | doi: 10.21228/M8RS3S |
| Project Title: | Metabolomics of Shark Bay stromatolites |
| Project Type: | global metabolomics |
| Project Summary: | screening two stromatolite forming mats for global profile of metabolites |
| Institute: | University of Florida |
| Department: | Microbiology and Cell Science |
| Laboratory: | Jamie Foster |
| Last Name: | Foster |
| First Name: | Jamie |
| Address: | Space Life Science Lab, 505 Odyssey Way, Merritt Island, FL 32953 |
| Email: | jfoster@ufl.edu |
| Phone: | 321-525-1047 |
| Funding Source: | NASA Astrobiology grant project 117514 |
Subject:
| Subject ID: | SU000594 |
| Subject Type: | bacterial microbial community |
| Subject Species: | Mixed |
| Species Group: | Other |
Factors:
Subject type: bacterial microbial community; Subject species: Mixed (Factor headings shown in green)
| mb_sample_id | local_sample_id | microbial community type |
|---|---|---|
| SA029957 | SCM8 | colloform stromatolite forming mat |
| SA029958 | SCM10 | colloform stromatolite forming mat |
| SA029959 | SCM2 | colloform stromatolite forming mat |
| SA029960 | SCM1 | colloform stromatolite forming mat |
| SA029961 | SCM7 | colloform stromatolite forming mat |
| SA029962 | SCM9 | colloform stromatolite forming mat |
| SA029963 | SCM6 | colloform stromatolite forming mat |
| SA029964 | SCM4 | colloform stromatolite forming mat |
| SA029965 | SCM5 | colloform stromatolite forming mat |
| SA029966 | SCM3 | colloform stromatolite forming mat |
| SA029967 | SPM5 | pustular stromatolite forming mat |
| SA029968 | SPM4 | pustular stromatolite forming mat |
| SA029969 | SPM1 | pustular stromatolite forming mat |
| SA029970 | SPM6 | pustular stromatolite forming mat |
| SA029971 | SPM3 | pustular stromatolite forming mat |
| SA029972 | SPM2 | pustular stromatolite forming mat |
| SA029973 | SPM10 | pustular stromatolite forming mat |
| SA029974 | SPM9 | pustular stromatolite forming mat |
| SA029975 | SPM8 | pustular stromatolite forming mat |
| SA029976 | SPM7 | pustular stromatolite forming mat |
| SA029977 | SSM6 | smooth stromatolite forming mat |
| SA029978 | SSM3 | smooth stromatolite forming mat |
| SA029979 | SSM2 | smooth stromatolite forming mat |
| SA029980 | SSM1 | smooth stromatolite forming mat |
| SA029981 | SSM4 | smooth stromatolite forming mat |
| SA029982 | SSM5 | smooth stromatolite forming mat |
| SA029983 | SSM9 | smooth stromatolite forming mat |
| SA029984 | SSM8 | smooth stromatolite forming mat |
| SA029985 | SSM7 | smooth stromatolite forming mat |
| SA029986 | SSM10 | smooth stromatolite forming mat |
| Showing results 1 to 30 of 30 |
Collection:
| Collection ID: | CO000588 |
| Collection Summary: | field collected samples from Shark Bay, Australia |
| Collection Protocol Filename: | Shark Bay microbial mats |
| Collection Protocol Comments: | collected using sterile scalpel and placed immediatelyl into RNAlater - frozen at -20C in field (2 weeks) - back in lab stored at -80C |
| Sample Type: | microbial community - biofilm |
| Collection Location: | Hamelin Pool, Shark Bay Western Australia |
| Collection Time: | 12PM |
| Storage Conditions: | RNALater |
| Collection Vials: | nalgene container |
Treatment:
| Treatment ID: | TR000608 |
| Treatment Summary: | no treatment; natural field collected samples |
Sample Preparation:
| Sampleprep ID: | SP000601 |
| Sampleprep Summary: | None |
| Sampleprep Protocol Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
Chromatography:
| Chromatography ID: | CH000625 |
| Methods Filename: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Scientific-Dionex Ultimate 3000 |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Flow Rate: | 0.350mL/min-0.600mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN000881 |
| Laboratory Name: | Garrett Lab |
| Analysis Type: | MS |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Scientific Q-Exactive Orbitrap |
| Chromatography ID: | CH000625 |
| Num Factors: | 3 |
| Num Metabolites: | 101 |
| Has Mz: | 1 |
| Rt Units: | Minutes |
| Units: | peak height |
| Analysis ID: | AN000882 |
| Laboratory Name: | Garrett Lab |
| Analysis Type: | MS |
| Analysis Protocol File: | Metabolomics_LCMSProtocol_plasma.pdf |
| Instrument Name: | Thermo Scientific Q-Exactive Orbitrap |
| Chromatography ID: | CH000625 |
| Num Factors: | 3 |
| Num Metabolites: | 60 |
| Has Mz: | 1 |
| Rt Units: | Minutes |
| Units: | peak height |
