Summary of Study ST000582
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000425. The data can be accessed directly via it's Project DOI: 10.21228/M84305 This work is supported by NIH grant, U2C- DK119886.
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| Study ID | ST000582 |
| Study Title | DBA2J Phospholipid Analysis |
| Study Summary | AH and TM samples were obtained from young-normotensive DBA/2J (3 and 7.5 months) and old-hypertensive DBA/2J mice (8-8.5, 10 and 12 month). Lipids were extracted using modified Bligh and Dyer method and subjected to mass spectrometric identification using appropriate class-specific lipid standards and ratiometric quantification. Corresponding aqueous phase (of extraction) protein concentrations were measured using Bradford method. |
| Institute | University of Miami |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | McKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136 |
| SBhattacharya@med.miami.edu | |
| Phone | 305-482-4103 |
| Submit Date | 2017-02-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000425 |
| Project DOI: | doi: 10.21228/M84305 |
| Project Title: | Phosphatidylethanolamine Analysis in Aqueous Humor and Trabecular Meshwork of DBA/2J mice |
| Project Summary: | Compared Phosphatidylethanolamine (PE) profiles within aqueous humor (AH) and trabecular meshwork (TM) of normotensive and hypertensive DBA/2J mice using direct infusion triple quadrupole mass spectrometry. |
| Institute: | University of Miami |
| Last Name: | Bhattacharya |
| First Name: | Sanjoy |
| Address: | McKnight Vision Research Building, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, 1638 NW 10th Avenue, Room 706A, Miami, Florida 33136 |
| Email: | Sbhattacharya@med.miami.edu |
| Phone: | 305-482-4103 |
Subject:
| Subject ID: | SU000605 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | DBA/2J |
| Species Group: | Mammals |
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Tissue Location | Sex | Phenotype |
|---|---|---|---|---|
| SA030777 | DBA2J 8-8.5M FAH4L3 | AH | Female | Hypertensive |
| SA030778 | DBA2J 10M FAHL4 | AH | Female | Hypertensive |
| SA030779 | DBA2J 12M FAHL1 | AH | Female | Hypertensive |
| SA030780 | DBA2J 7.5M FAHL4 | AH | Female | Normotensive |
| SA030781 | DBA2J 3M FAHL1 | AH | Female | Normotensive |
| SA030782 | DBA2J 8-8.5M MAH4L3 | AH | Male | Hypertensive |
| SA030783 | DBA2J 10 MAHL3 | AH | Male | Hypertensive |
| SA030784 | DBA2J 12M MAHL1 | AH | Male | Hypertensive |
| SA030785 | DBA2J 3M MAHL1 | AH | Male | Normotensive |
| SA030786 | DBA2J 7.5M MAH2L1 | AH | Male | Normotensive |
| SA030787 | DBA2J 8-8.5M FTML2 | TM | Female | Hypertensive |
| SA030788 | DBA2J 10M FTML3 | TM | Female | Hypertensive |
| SA030789 | DBA2J 12M FTML1 | TM | Female | Hypertensive |
| SA030790 | DBA2J 7.5M FTML3 | TM | Female | Normotensive |
| SA030791 | DBA2J 3M FTML4 | TM | Female | Normotensive |
| SA030792 | DBA2J 10M MTML3 | TM | Male | Hypertensive |
| SA030793 | DBA2J 8-8.5M MTM3L3 | TM | Male | Hypertensive |
| SA030794 | DBA2J 12M MTML1 | TM | Male | Hypertensive |
| SA030795 | DBA2J 7.5M MTM2L1 | TM | Male | Normotensive |
| SA030796 | DBA2J 3M MTML1 | TM | Male | Normotensive |
| Showing results 1 to 20 of 20 |
Collection:
| Collection ID: | CO000599 |
| Collection Summary: | DBA/2J mice were anesthetized by an intraperitoneal injection of a cocktail of Ketamine and Xylazine. A drop of tetracaine hydrochloride ophthalmic solution (0.5%, Bausch & Lomb, USA) was used to anesthetize the ocular surface prior to AH procurement. Normotensive and hypertensive AH was obtained from the anterior chamber of DBA/2J mice via paracentesis using a syringe (catalog no. 7635-01 Hamilton, Reno, Nevada) with a small 33 gauge removable needle (701RN, catalog no. 7803-05 Hamilton). TM samples were obtained through surgical removal after euthanization with carbon dioxide and adhering to animal procedures approved by the IACUC. |
| Sample Type: | Eye tissue |
Treatment:
| Treatment ID: | TR000619 |
| Treatment Summary: | No treatment was performed on the mice. |
Sample Preparation:
| Sampleprep ID: | SP000612 |
| Sampleprep Summary: | A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method. |
Chromatography:
| Chromatography ID: | CH000636 |
| Instrument Name: | TSQ Quantum Access Max |
| Column Name: | None |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN000893 |
| Analysis Type: | MS |
| Instrument Name: | TSQ Quantum Access Max |
| Chromatography ID: | CH000636 |
| Num Factors: | 8 |
| Num Metabolites: | 163 |
| Units: | pmol/ ug of protein |
