Summary of Study ST000615

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000447. The data can be accessed directly via it's Project DOI: 10.21228/M88P55 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000615
Study TitleGC/MS measurement of sarcosine in urine samples
Study SummaryGC/MS measurement of sarcosine in urine samples from prostate cancer and case control patients in a group of ancestry verified African American and European Americans.
Institute
Baylor College of Medicine
Last NameSreekumar
First NameArun
AddressOne Baylor Plaza, Houston, TX, 77030, USA
EmailArun.Sreekumar@bcm.edu
Phone713-798-3305
Submit Date2017-05-19
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Arun Sreekumar Arun Sreekumar
https://dx.doi.org/10.21228/M88P55
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000447
Project DOI:doi: 10.21228/M88P55
Project Title:Prostate cancer disparity study on urine
Project Summary:LC/MS-MS measurement of inosine to adenosine ratio as well as GC/MS measurement of sarcosine relative values in urine samples from
Institute:Baylor College of Medicine
Last Name:Sreekumar
First Name:Arun
Address:One Baylor Plaza, Houston, TX, 77030, USA
Email:Arun.Sreekumar@bcm.edu
Phone:713-798-3305

Subject:

Subject ID:SU000638
Subject Type:Humans
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Humans; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Diagnosis RACE
SA03408018112Case control -
SA03408118106Case control -
SA03408218137Case control -
SA03408318140Case control -
SA03408418154Case control -
SA03408518102Case control -
SA03408618091Case control -
SA03408718056Case control -
SA03408818062Case control -
SA03408918072Case control -
SA03409018077Case control -
SA03409118156Case control -
SA03409218174Case control -
SA03409318415Case control -
SA03409418277Case control -
SA03409518469Case control -
SA03409618489Case control -
SA03409718661Case control -
SA03409818265Case control -
SA03409918261Case control -
SA03410018054Case control -
SA03410118225Case control -
SA03410218240Case control -
SA03410318250Case control -
SA03410418162Case control -
SA03410518098Case control -
SA03410617962Case control -
SA03410718004Case control -
SA03410818010Case control -
SA03410917902Case control -
SA03411017893Case control -
SA03411118035Case control -
SA03411217848Case control -
SA03411317879Case control -
SA03411418013Case control -
SA03411517817Case control -
SA03411618014Case control -
SA03411718030Case control -
SA03411817122Prostate cancer African American
SA03411917234Prostate cancer African American
SA03412018331Prostate cancer African American
SA03412118097Prostate cancer African American
SA03412216710Prostate cancer African American
SA03412318882Prostate cancer African American
SA03412419049Prostate cancer African American
SA03412518998Prostate cancer African American
SA03412618901Prostate cancer African American
SA03412718887Prostate cancer African American
SA03412818850Prostate cancer African American
SA03412918317Prostate cancer African American
SA03413018020Prostate cancer African American
SA03413118064Prostate cancer African American
SA03413218073Prostate cancer African American
SA03413317985Prostate cancer African American
SA03413415298Prostate cancer African American
SA03413517891Prostate cancer African American
SA03413617897Prostate cancer African American
SA03413718175Prostate cancer African American
SA03413818918Prostate cancer Caucasian
SA03413918018Prostate cancer Caucasian
SA03414018262Prostate cancer Caucasian
SA03414118083Prostate cancer Caucasian
SA03414219034Prostate cancer Caucasian
SA03414315862Prostate cancer Caucasian
SA03414419169Prostate cancer Caucasian
SA03414517721Prostate cancer Caucasian
SA03414617587Prostate cancer Caucasian
SA03414717585Prostate cancer Caucasian
SA03414818542Prostate cancer Caucasian
SA03414917024Prostate cancer Caucasian
SA03415018063Prostate cancer Caucasian
SA03415118613Prostate cancer Caucasian
SA03415218061Prostate cancer Caucasian
SA03415318742Prostate cancer Caucasian
SA03415418734Prostate cancer Caucasian
SA03415518830Prostate cancer Caucasian
Showing results 1 to 76 of 76

Collection:

Collection ID:CO000632
Collection Summary:Urine from ancestry typed AA and EA prostate cancer patients were used for this analysis. Urine specimens were spun and the supernatant was stored at −70°C until analysis.
Sample Type:Urine

Treatment:

Treatment ID:TR000652
Treatment Summary:NA

Sample Preparation:

Sampleprep ID:SP000645
Sampleprep Summary:Urine supernatants were diluted with methanol containing spiked labeled internal standards (d3-sarcosine and d3-alanine), and extracted overnight at 4°C, using 1:1 molar ratio of water/chloroform at room temperature, with constant shaking. The aqueous methanolic layer was recovered, dried and azeotroped twice by adding 100 mL dimethylformamide (DMF), mixed and dried using a speedvac for 30 minutes. Following this, 100 mL of DMF and N-methyl-N-tert-butylmethylsilyltrifluoroacetamide (MtBSTFA) + 1% t-butyl-dimethylchlorosilane were added and incubated at 60°C for 1 hr. The samples were resuspended in ethyl acetate and injected into a GC-MS.

Chromatography:

Chromatography ID:CH000672
Chromatography Summary:During the course of the run, temperature was ramped from 40° to 300° C in a 16 minute period.
Instrument Name:Agilent
Column Name:Agilent DB5-MS (15m)
Chromatography Type:GC

Analysis:

Analysis ID:AN000944
Analysis Type:MS
Instrument Name:Agilent
Chromatography ID:CH000672
Num Factors:3
Num Metabolites:1
Rt Units:Minutes
Units:Area
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