Summary of Study ST000662
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000465. The data can be accessed directly via it's Project DOI: 10.21228/M8Z60N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000662 |
| Study Title | Lipidomics of hormone-sensitive lipase variants |
| Study Type | MS analysis |
| Study Summary | The samples are frozen biopsies of whilte subcutaneous adipose tissues (50 mg). Samples were collected from 4 hormone-sensitive lipase (HSL) WT patients (adipose insulin sensitive), 3 HSL heterozygote patients and 1 HSL KO patient. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 3 |
| Total Subjects | 8 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000465 |
| Project DOI: | doi: 10.21228/M8Z60N |
| Project Title: | Hormone-sensitive lipase (HSL) knock-out |
| Project Type: | MS analysis |
| Project Summary: | Lipidomics in control, HSL ID, HSL DD |
| Institute: | University of Maryland |
| Department: | Medicine |
| Laboratory: | Sztalryd-Woodle Lab |
| Last Name: | Sztalryd-Woodle |
| First Name: | Carole |
| Address: | Baltimore, MD |
| Email: | csztalry@grecc.umaryland.edu |
| Phone: | 410-706-4047 |
Subject:
| Subject ID: | SU000685 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Genotype |
|---|---|---|
| SA038150 | S00014864 | HSL heterozygote |
| SA038151 | S00014863 | HSL heterozygote |
| SA038152 | S00014862 | HSL heterozygote |
| SA038149 | S00014865 | HSL KO |
| SA038153 | S00014859 | WT |
| SA038154 | S00014860 | WT |
| SA038155 | S00014861 | WT |
| SA038156 | S00014858 | WT |
| Showing results 1 to 8 of 8 |
Collection:
| Collection ID: | CO000679 |
| Collection Summary: | none |
| Sample Type: | Adipose tissue |
Treatment:
| Treatment ID: | TR000699 |
| Treatment Summary: | none |
Sample Preparation:
| Sampleprep ID: | SP000692 |
| Sampleprep Summary: | none |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Chromatography:
| Chromatography ID: | CH000725 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001010 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000725 |
| Num Factors: | 3 |
| Num Metabolites: | 249 |
| Rt Units: | Minutes |
| Units: | peak area |
| Analysis ID: | AN001011 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000725 |
| Num Factors: | 3 |
| Num Metabolites: | 564 |
| Rt Units: | Minutes |
| Units: | peak area |
