Summary of Study ST000666
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000469. The data can be accessed directly via it's Project DOI: 10.21228/M8F61C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000666 |
| Study Title | Rat Rotator Cuff Lipidomics |
| Study Type | MS analysis |
| Study Summary | Rats were subjected to bilateral rotator cuff tears of the right and left supraspinatus muscle. Muscles were harvested from each shoulder at 0, 10, 30, or 60 days post surgery. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000469 |
| Project DOI: | doi: 10.21228/M8F61C |
| Project Title: | Rat Rotator Cuff Lipidomics Study |
| Project Type: | MS analysis |
| Project Summary: | Shotgun Lipidomics of samples obtained from various timepoints of rotator cuff injury |
| Institute: | University of Michigan |
| Department: | Orthopaedic Surgery |
| Laboratory: | Mendias Lab |
| Last Name: | Mendias |
| First Name: | Christopher |
| Address: | Ann Arbor, MI |
| Email: | cmendias@umich.edu |
| Phone: | 734-764-3250 |
Subject:
| Subject ID: | SU000689 |
| Subject Type: | RAT |
| Subject Species: | Rattus norvegicus |
| Taxonomy ID: | 10116 |
| Species Group: | Mammals |
Factors:
Subject type: RAT; Subject species: Rattus norvegicus (Factor headings shown in green)
| mb_sample_id | local_sample_id | Side | TIME_DAYS |
|---|---|---|---|
| SA038384 | S00016773 | L | - |
| SA038385 | S00016779 | L | - |
| SA038386 | S00016777 | L | - |
| SA038387 | S00016781 | L | - |
| SA038388 | S00016775 | L | - |
| SA038389 | S00016785 | L | 10 |
| SA038390 | S00016783 | L | 10 |
| SA038391 | S00016791 | L | 10 |
| SA038392 | S00016787 | L | 10 |
| SA038393 | S00016789 | L | 10 |
| SA038394 | S00016797 | L | 30 |
| SA038395 | S00016801 | L | 30 |
| SA038396 | S00016799 | L | 30 |
| SA038397 | S00016795 | L | 30 |
| SA038398 | S00016793 | L | 30 |
| SA038399 | S00016805 | L | 60 |
| SA038400 | S00016803 | L | 60 |
| SA038401 | S00016807 | L | 60 |
| SA038402 | S00016809 | L | 60 |
| SA038403 | S00016811 | L | 60 |
| SA038404 | S00016778 | R | - |
| SA038405 | S00016774 | R | - |
| SA038406 | S00016780 | R | - |
| SA038407 | S00016776 | R | - |
| SA038408 | S00016782 | R | - |
| SA038409 | S00016784 | R | 10 |
| SA038410 | S00016792 | R | 10 |
| SA038411 | S00016788 | R | 10 |
| SA038412 | S00016790 | R | 10 |
| SA038413 | S00016786 | R | 10 |
| SA038414 | S00016798 | R | 30 |
| SA038415 | S00016794 | R | 30 |
| SA038416 | S00016796 | R | 30 |
| SA038417 | S00016800 | R | 30 |
| SA038418 | S00016802 | R | 30 |
| SA038419 | S00016804 | R | 60 |
| SA038420 | S00016812 | R | 60 |
| SA038421 | S00016808 | R | 60 |
| SA038422 | S00016810 | R | 60 |
| SA038423 | S00016806 | R | 60 |
| Showing results 1 to 40 of 40 |
Collection:
| Collection ID: | CO000683 |
| Collection Summary: | - |
| Sample Type: | Muscle |
Treatment:
| Treatment ID: | TR000703 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000696 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Chromatography:
| Chromatography ID: | CH000729 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001018 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000729 |
| Num Factors: | 8 |
| Num Metabolites: | 333 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
| Analysis ID: | AN001019 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000729 |
| Num Factors: | 8 |
| Num Metabolites: | 568 |
| Rt Units: | Minutes |
| Units: | peak area normalized |