Summary of Study ST000669

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000472. The data can be accessed directly via it's Project DOI: 10.21228/M82028 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000669
Study TitleCharacterization of Retinal Exudates in Coats Disease - lipid profile
Study TypeMS analysis
Study SummaryThe aim of our study is to characterize the retinal lipid exudates through lipidomic assays. Lipidomic analysis will quantify and identify the retinal lipid exudates and provide a `lipid profile? of the exudates. The characterization of the retinal lipids will help in further understanding the pathogenesis of Coat?s disease. It may allow us to identify and therapeutically target a metabolic pathway to prevent retinal exudation abnormal in Coat?s disease.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-05
Num Groups11
Total Subjects4
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M82028
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000472
Project DOI:doi: 10.21228/M82028
Project Title:Characterization of Retinal Exudates in Coats Disease
Project Type:MS analysis
Project Summary:The aim of our study is to characterize the retinal lipid exudates through lipidomic assays
Institute:University of Michigan
Department:Ophthalmology and Visual Sciences
Laboratory:Besirli Lab
Last Name:Besirli
First Name:Cagri
Address:Ann Arbor, MI
Email:cbesirli@umich.edu
Phone:734-615-6099

Subject:

Subject ID:SU000692
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Mammals

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Disease Matrix Sample type
SA038496S00019676AS-Control-P Plasma Plasma
SA038497S00019675AS-Control-S SRF Fluids and Secretions
SA038498S00019672Coats CB Fluids and Secretions
SA038499S00019673Coats CB Fluids and Secretions
SA038500S00019674JB-Coats-P Plasma Plasma
Showing results 1 to 5 of 5

Collection:

Collection ID:CO000686
Collection Summary:-
Sample Type:Retina

Treatment:

Treatment ID:TR000706
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000699
Sampleprep Summary:-
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Chromatography:

Chromatography ID:CH000732
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN001024
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000732
Num Factors:4
Num Metabolites:149
Rt Units:Minutes
Units:peak area
  
Analysis ID:AN001025
Analysis Type:MS
Analysis Protocol File:A004_-_Shotgun_Lipidomics.pdf
Instrument Name:TripleTOF 5600+ (Absciex)
Chromatography ID:CH000732
Num Factors:4
Num Metabolites:337
Rt Units:Minutes
Units:peak area
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