Summary of Study ST000678
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000480. The data can be accessed directly via it's Project DOI: 10.21228/M8102Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000678 |
| Study Title | Functional genomics study on Non Alcoholic fatty liver disease (NAFLD) |
| Study Type | MS analysis |
| Study Summary | In this experiment, vector , GCKR (WT), GCKR(P446L), PPP1R3B, TM6SF2 (WT and E167K) overexpression stable HuH-7_Lok cell lines as well as Non-target, GCKR shRNA (52621), PPP1R3B shRNA (2640), TM6SF2 shRNA (382426) knockdown HuH-7_Lok cell lines are used to do the lipidomics experiments 3 X 100 mm dishes of cells were seeded (1x106 cells/dish) for each cell line and incubated at 37? C for 24 hours, then replaced medium with delipidated serum DMEM medium. After 24 hours, treated with BSA-OA (200?M) for another 24 hours before the collection of cells Add 4mL/dish 50 mM ammonium acetate wash cells and then quench cells by adding liquid nitrogen covering all the surface of dish. Store the cell samples in -80?C freezer. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 6 |
| Total Subjects | 1 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000480 |
| Project DOI: | doi: 10.21228/M8102Z |
| Project Title: | Functional genomics study on Non Alcoholic fatty liver disease (NAFLD) |
| Project Type: | MS analysis |
| Project Summary: | In this study, we determine the genetic mechanism of action by which variants near PNPLA3 and PPP1R3B exert their effects on promoting NAFLD |
| Institute: | University of Michigan |
| Department: | Internal Medicine |
| Laboratory: | Speliotes Lab |
| Last Name: | Speliotes |
| First Name: | Elizabeth |
| Address: | Ann Arbor, MI |
| Email: | espeliot@umich.edu |
| Phone: | 734-647-2964 |
Subject:
| Subject ID: | SU000701 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Mammals |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Construct |
|---|---|---|
| SA038944 | S00026025 | GCKR(P446L) |
| SA038945 | S00026024 | GCKR(P446L) |
| SA038946 | S00026023 | GCKR(P446L) |
| SA038947 | S00026021 | GCKR(WT) |
| SA038948 | S00026020 | GCKR(WT) |
| SA038949 | S00026022 | GCKR(WT) |
| SA038950 | S00026026 | NT |
| SA038951 | S00026027 | NT |
| SA038952 | S00026028 | NT |
| SA038953 | S00026029 | PPP1R3B_2640 |
| SA038954 | S00026030 | PPP1R3B_2640 |
| SA038955 | S00026031 | PPP1R3B_2640 |
| SA038956 | S00026017 | PPP1R3B_3 |
| SA038957 | S00026018 | PPP1R3B_3 |
| SA038958 | S00026019 | PPP1R3B_3 |
| SA038959 | S00026015 | Vector |
| SA038960 | S00026016 | Vector |
| SA038961 | S00026014 | Vector |
| Showing results 1 to 18 of 18 |
Collection:
| Collection ID: | CO000695 |
| Collection Summary: | - |
| Sample Type: | Cultured cells |
Treatment:
| Treatment ID: | TR000715 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000708 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Chromatography:
| Chromatography ID: | CH000741 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
Analysis:
| Analysis ID: | AN001042 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000741 |
| Num Factors: | 6 |
| Num Metabolites: | 192 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
| Analysis ID: | AN001043 |
| Analysis Type: | MS |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| Instrument Name: | TripleTOF 5600+ (Absciex) |
| Chromatography ID: | CH000741 |
| Num Factors: | 6 |
| Num Metabolites: | 259 |
| Rt Units: | Minutes |
| Units: | peak area normalized |
